RGD Reference Report - Cloning of cDNA encoding rat spermatidal protein TP2 and expression in Escherichia coli. - Rat Genome Database

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Cloning of cDNA encoding rat spermatidal protein TP2 and expression in Escherichia coli.

Authors: Meetei, AR  Rao, MR 
Citation: Meetei AR and Rao MR, Protein Expr Purif 1996 Dec;8(4):409-15.
RGD ID: 634495
Pubmed: PMID:8954887   (View Abstract at PubMed)
DOI: DOI:10.1006/prep.1996.0118   (Journal Full-text)

Rat spermatidal protein TP2 is a basic nuclear protein containing two atoms of zinc bound per molecule. We report here cloning of complementary DNA encoding rat TP2 by the RT-PCR method. The nucleotide sequence of cloned TP2 cDNA differs at a few positions from the sequence already reported in the literature. We have cloned rat TP2 cDNA into the expression vector pTrc 99A. Upon induction with 1 mM IPTG, there was a low level of expression of TP2 which could be recovered in the soluble form. Recombinant TP2 was purified from the soluble form. Recombinant TP2 was purified from the soluble extract of E. coli using nickel-agarose and heparin-agarose chromatography and was shown to be identical to native rat TP2 as revealed by immunoblotting with anti-rat TP2 antibodies and radioactive 65Zn-blotting.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
zinc ion binding  IDA 634495 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tnp2  (transition protein 2)


Additional Information