RGD Reference Report - Up-regulated uridine kinase gene identified by RLCS in the ventral horn after crush injury to rat sciatic nerves. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Up-regulated uridine kinase gene identified by RLCS in the ventral horn after crush injury to rat sciatic nerves.

Authors: Yuh, I  Yaoi, T  Watanabe, S  Okajima, S  Hirasawa, Y  Fushiki, S 
Citation: Yuh I, etal., Biochem Biophys Res Commun 1999 Dec 9;266(1):104-9.
RGD ID: 634248
Pubmed: (View Article at PubMed) PMID:10581173
DOI: Full-text: DOI:10.1006/bbrc.1999.1781

Rat sciatic nerve crush injury is one of the models commonly employed for studying the mechanisms of nerve regeneration. In this study, we analyzed the temporal change of gene expression after injury in this model, to elucidate the molecular mechanisms involved in nerve regeneration. First, a cDNA analysis method, Restriction Landmark cDNA Scanning (RLCS), was applied to cells in the ventral horn of the spinal cord during a 7-day period after the crush injury. A total of 1991 cDNA species were detected as spots on gels, and 37 of these were shown to change after the injury. Temporally changed patterns were classified into three categories: the continuously up-regulated type (10 species), the transiently up-regulated type (22 species), and the down-regulated type (5 species). These complex patterns of gene expression demonstrated after the injury suggest that precise regulation in molecular pathways is required for accomplishing nerve regeneration. Secondly, the rat homologue of uridine kinase gene was identified as one of the up-regulated genes. Northern blot analysis on rat ventral horn tissue and brain revealed that the UK gene had three transcripts with different sizes (4.3, 1. 4, and 1.35 kb, respectively). All of the transcripts, especially the 4.3 kb one, were up-regulated mainly in a bimodal fashion during the 28-day period after the injury. The RLCS method that we employed in the present study shows promise as a means to fully analyze molecular changes in nerve regeneration in detail.

Annotation

Disease Annotations    

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Uck1  (uridine-cytidine kinase 1)
Uck2  (uridine-cytidine kinase 2)

Genes (Mus musculus)
Uck1  (uridine-cytidine kinase 1)

Genes (Homo sapiens)
UCK1  (uridine-cytidine kinase 1)

Objects referenced in this article
0 Cmpk1 cytidine/uridine monophosphate kinase 1 All species
0 Cmpk1 cytidine monophosphate (UMP-CMP) kinase 1 All species
0 CMPK1 cytidine/uridine monophosphate kinase 1 All species

Additional Information