RGD Reference Report - Krp1, a novel kelch related protein that is involved in pseudopod elongation in transformed cells. - Rat Genome Database

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Krp1, a novel kelch related protein that is involved in pseudopod elongation in transformed cells.

Authors: Spence, HJ  Johnston, I  Ewart, K  Buchanan, SJ  Fitzgerald, U  Ozanne, BW 
Citation: Spence HJ, etal., Oncogene 2000 Mar 2;19(10):1266-76.
RGD ID: 634002
Pubmed: (View Article at PubMed) PMID:10713668
DOI: Full-text: DOI:10.1038/sj.onc.1203433

We have previously shown that the transcription factor AP-1 regulates the expression of genes which allow neoplastically transformed rat fibroblasts to become invasive. Searches for further AP-1 target genes led to the identification of a gene encoding a novel rat kelch family member, named kelch related protein 1 (Krp1). Kelch family members are characterized by a series of repeats at their carboxyl terminus and a BTB/POZ domain near their amino terminus. Rat Krp1 has a primarily cytoplasmic localization, and a small fraction appears to accumulate and co-localize with F-actin at membrane ruffle-like structures in the tips of pseudopodia. Overexpression of Krp1 in transformed rat fibroblasts led to the formation of dramatically elongated pseudopodia, while expression of truncated Krp1 polypeptides resulted in a reduction in the length of pseudopodia. We propose that the transformation-specific expression of Krp1 is required for pseudopod elongation, which are structures that are required for cell motility and invasion.

Annotation

Gene Ontology Annotations    

Biological Process

Cellular Component
cytoplasm  (IDA)
pseudopodium  (IDA)

Objects Annotated

Genes (Rattus norvegicus)
Klhl41  (kelch-like family member 41)


Additional Information