RGD Reference Report - The neural cell recognition molecule neurofascin interacts with syntenin-1 but not with syntenin-2, both of which reveal self-associating activity. - Rat Genome Database

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The neural cell recognition molecule neurofascin interacts with syntenin-1 but not with syntenin-2, both of which reveal self-associating activity.

Authors: Koroll, M  Rathjen, FG  Volkmer, H 
Citation: Koroll M, etal., J Biol Chem 2001 Apr 6;276(14):10646-54.
RGD ID: 633988
Pubmed: (View Article at PubMed) PMID:11152476
DOI: Full-text: DOI:10.1074/jbc.M010647200

Neurofascin belongs to the L1 subgroup of the immunoglobulin superfamily of cell adhesion molecules and is implicated in axonal growth and fasciculation. We used yeast two-hybrid screening to identify proteins that interact with neurofascin intracellularly and therefore might link it to trafficking, spatial targeting, or signaling pathways. Here, we demonstrate that rat syntenin-1, previously published as syntenin, mda-9, or TACIP18 in human, is a neurofascin-binding protein that exhibits a wide-spread tissue expression pattern with a relative maximum in brain. Syntenin-1 was found not to interact with other vertebrate members of the L1 subgroup such as L1 itself or NrCAM. We confirmed the specificity of the neurofascin-syntenin-1 interaction by ligand-overlay assay, surface plasmon resonance analysis, and colocalization of both proteins in heterologous cells. The COOH terminus of neurofascin was mapped to interact with the second PDZ domain of syntenin-1. Furthermore, we isolated syntenin-2 that may be expressed in two isoforms. Despite their high sequence similarity to syntenin-1, syntenin-2alpha, which interacts with neurexin I, and syntenin-2beta do not bind to neurofascin or several other transmembrane proteins that are binding partners of syntenin-1. Finally, we report that syntenin-1 and -2 both form homodimers and can interact with each other.

Annotation

Gene Ontology Annotations    

Cellular Component
cytoskeleton  (IDA)

Objects Annotated

Genes (Rattus norvegicus)
L1cam  (L1 cell adhesion molecule)
Sdcbp  (syndecan binding protein)
Snap25  (synaptosome associated protein 25)
Trim9  (tripartite motif-containing 9)

Objects referenced in this article
0 Nfasc neurofascin All species
0 NRXN1 neurexin 1 All species
0 SDCBP2 syndecan binding protein 2 All species

Additional Information