RGD Reference Report - Mammalian Sly1 regulates syntaxin 5 function in endoplasmic reticulum to Golgi transport. - Rat Genome Database

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Mammalian Sly1 regulates syntaxin 5 function in endoplasmic reticulum to Golgi transport.

Authors: Dascher, C  Balch, WE 
Citation: Dascher C and Balch WE, J Biol Chem 1996 Jul 5;271(27):15866-9.
RGD ID: 633773
Pubmed: PMID:8663406   (View Abstract at PubMed)

Members of the syntaxin gene family are components of protein complexes which regulate vesicle docking and/or fusion during transport of cargo through the secretory pathway of eukaryotic cells. We have previously demonstrated that syntaxin 5 is specifically required for endoplasmic reticulum to Golgi transport (Dascher, C., Matteson, J., and Balch, W. E.(1994) J. Biol. Chem. 269, 29363-29366). To extend these observations we have now cloned a protein from rat liver membranes which forms a native complex with syntaxin 5. We demonstrate that this protein is the mammalian homologue to yeast Sly1p, previously identified as a protein which genetically and biochemically interacts with the small GTPase Ypt1p and Sed5p, proteins involved in docking/fusion in the early secretory pathway of yeast. Using transient expression we find that overexpression of rat liver Sly1 (rSly1) can neutralize the dominant negative effects of excess syntaxin 5 on endoplasmic reticulum to Golgi transport. These results suggest that rSly1 functions to positively regulate syntaxin 5 function.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
endoplasmic reticulum to Golgi vesicle-mediated transport  TAS 633773 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
syntaxin binding  TAS 633773 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Scfd1  (sec1 family domain containing 1)


Additional Information