RGD Reference Report - Regulatory interaction of phosducin-like protein with the cytosolic chaperonin complex. - Rat Genome Database

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Regulatory interaction of phosducin-like protein with the cytosolic chaperonin complex.

Authors: McLaughlin, JN  Thulin, CD  Hart, SJ  Resing, KA  Ahn, NG  Willardson, BM 
Citation: McLaughlin JN, etal., Proc Natl Acad Sci U S A 2002 Jun 11;99(12):7962-7.
RGD ID: 633730
Pubmed: PMID:12060742   (View Abstract at PubMed)
PMCID: PMC123003   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.112075699   (Journal Full-text)

Phosducin and phosducin-like protein (PhLP) bind G protein betagamma subunits and regulate their activity. This report describes a previously uncharacterized binding partner unique to PhLP that was discovered by coimmunoprecipitation coupled with mass spectrometric identification. Chaperonin containing tailless complex polypeptide 1 (CCT), a cytosolic chaperone responsible for the folding of many cellular proteins, binds PhLP with a stoichiometry of one PhLP per CCT complex. Unlike protein-folding substrates of CCT, which interact only in their nonnative conformations, PhLP binds in its native state. Native PhLP competes directly for binding of protein substrates of CCT and thereby inhibits CCT activity. Overexpression of PhLP inhibited the ability of CCT to fold newly synthesized beta-actin by 80%. These results suggest that the interaction between PhLP and CCT may be a means to regulate CCT-dependent protein folding or alternatively, to control the availability of PhLP to modulate G protein signaling.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of protein refolding  IDA 633730 RGD 
protein folding  IDA 633730 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein-containing complex binding  IDA 633730 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pdcl  (phosducin like)

Additional Information