RGD Reference Report - Molecular cloning of neonate/infant-specific pepsinogens from rat stomach mucosa and their expressional change during development. - Rat Genome Database

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Molecular cloning of neonate/infant-specific pepsinogens from rat stomach mucosa and their expressional change during development.

Authors: Kageyama, T  Ichinose, M  Tsukada-Kato, S  Omata, M  Narita, Y  Moriyama, A  Yonezawa, S 
Citation: Kageyama T, etal., Biochem Biophys Res Commun 2000 Jan 27;267(3):806-12.
RGD ID: 633656
Pubmed: PMID:10673373   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1999.2047   (Journal Full-text)

To clarify the nature of rat neonate/infant-specific pepsinogens, we carried out their purification and molecular cloning. Prochymosin was found to be the major neonatal pepsinogen. The general proteolytic activity of its active form, chymosin, was, however, lower than those of pepsins A and C which are predominant in adult animals. Molecular cloning of rat prochymosin cDNA was achieved along with cDNA for another neonate-specific pepsinogen, pepsinogen F, although determination of pepsinogen F in neonatal gastric mucosa was unsuccessful, presumably due to its lack of proteolytic activity or different proteolytic specificity. Northern blot analysis confirmed that genes for prochymosin and pepsinogen F are expressed only at neonatal/infant stages and the switching of gene expression to that of pepsinogen C occurred at late infant stages. A phylogenetic tree based on nucleotide sequences showed clearly that pepsinogens fall into four major groups, namely prochymosin and pepsinogen F of the neonate/infant and pepsinogens A and C of adult animals. Although, to date, prochymosin and pepsinogen F were believed to be expressed in only a limited number of mammals, the present results suggest that they might be expressed at the neonatal/infant stage in a variety of mammals.

Objects referenced in this article
Gene Cym chymosin Rattus norvegicus
Gene Pga5 pepsinogen A5 Rattus norvegicus

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