RGD Reference Report - Cloning of a lymphatic peptide/histidine transporter. - Rat Genome Database

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Cloning of a lymphatic peptide/histidine transporter.

Authors: Sakata, K  Yamashita, T  Maeda, M  Moriyama, Y  Shimada, S  Tohyama, M 
Citation: Sakata K, etal., Biochem J 2001 May 15;356(Pt 1):53-60.
RGD ID: 633591
Pubmed: PMID:11336635   (View Abstract at PubMed)
PMCID: PMC1221811   (View Article at PubMed Central)

Although peptide transport across the plasma membrane has been characterized well in the kidney and the intestine, the functional relevance of this transport in other organs has not been addressed. Here we report the cloning of a cDNA for a novel peptide/histidine transporter found in the rat (rPHT2), whose mRNA is expressed mainly in the lymphatic system. rPHT2 encodes a protein of 582 amino acids and showed 49% identity with the brain PHT (PHT1) [Yamashita, Shimada, Guo, Sato, Kohmura, Hayakawa, Takagi and Tohyama (1997) J. Biol. Chem. 272, 10205-10211]. rPHT2 mRNA was abundant in lung, spleen and thymus, and detected faintly in brain, liver, adrenal gland and heart by Northern-blot analysis and reverse transcriptase PCR (RT-PCR). Intense signals for the gene were found in immunocytes using in situ hybridization. Ectopic expression of rPHT2 protein in HEK-293T cells and BHK cells was not found on the cell surface, but was found on the lysosomal membrane using light- and electron-microscopic analysis. Recombinant rPHT2 protein reconstituted into liposomes showed proton-dependent transport activity with histidine and histidyl-leucine. These findings suggest that rPHT2 is involved in the protein catabolic pathway in the lymphatic system.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein catabolic process  NAS 633591 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lysosomal membrane  IDA 633591 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
proton-dependent oligopeptide secondary active transmembrane transporter activity  TAS 633591 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc15a3  (solute carrier family 15 member 3)

Objects referenced in this article
Gene SLC15A3 solute carrier family 15 member 3 Homo sapiens

Additional Information