RGD Reference Report - Actopaxin is phosphorylated during mitosis and is a substrate for cyclin B1/cdc2 kinase. - Rat Genome Database

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Actopaxin is phosphorylated during mitosis and is a substrate for cyclin B1/cdc2 kinase.

Authors: Curtis, M  Nikolopoulos, SN  Turner, CE 
Citation: Curtis M, etal., Biochem J 2002 Apr 15;363(Pt 2):233-42.
RGD ID: 633557
Pubmed: PMID:11931650   (View Abstract at PubMed)
PMCID: PMC1222471   (View Article at PubMed Central)

Prior to cell division, normal adherent cells adopt a round morphology that is associated with a loss of actin stress fibres and disassembly of focal adhesions. In this study, we investigate the mitotic phosphorylation of the recently described paxillin and actin-binding focal-adhesion protein actopaxin [Nikolopoulos and Turner (2000) J. Cell Biol. 151, 1435-1448]. Actopaxin is comprised of an N-terminus containing six putative cdc2 phosphorylation sites and a C-terminus consisting of tandem calponin homology domains. Here we show that the N-terminus of actopaxin is phosphorylated by cyclin B1/cdc2 kinase in vitro and that this region of actopaxin precipitates cdc2 kinase activity from mitotic lysates. Actopaxin exhibits reduced electrophoretic mobility during mitosis that is dependent on phosphorylation within the first two consensus cdc2 phosphorylation sites. Finally, as cells progress from mitosis to G(1) there is an adhesion-independent dephosphorylation of actopaxin, suggesting that actopaxin dephosphorylation precedes cell spreading and the reformation of focal adhesions. Taken together, these results suggest a role for cyclin B1/cdc2-dependent phosphorylation of actopaxin in regulating actin cytoskeleton reorganization during cell division.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
actin cytoskeleton organization  IDA 633557 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
focal adhesion  NAS 633557 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Parva  (parvin, alpha)


Additional Information