RGD Reference Report - A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways. - Rat Genome Database

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A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways.

Authors: Meyer, HH  Shorter, JG  Seemann, J  Pappin, D  Warren, G 
Citation: Meyer HH, etal., EMBO J 2000 May 15;19(10):2181-92.
RGD ID: 633500
Pubmed: PMID:10811609   (View Abstract at PubMed)
PMCID: PMC384367   (View Article at PubMed Central)
DOI: DOI:10.1093/emboj/19.10.2181   (Journal Full-text)

The AAA-ATPase, p97/Cdc48p, has been implicated in many different pathways ranging from membrane fusion to ubiquitin-dependent protein degradation. Binding of the p47 complex directs p97 to act in the post-mitotic fusion of Golgi membranes. We now describe another binding complex comprising mammalian Ufd1 and Npl4. Yeast Ufd1p is required for ubiquitin-dependent protein degradation whereas yeast Npl4p has been implicated in nuclear transport. In rat liver cytosol, Ufd1 and Npl4 form a binary complex, which exists either alone or bound to p97. Ufd1/Npl4 competes with p47 for binding to p97 and so inhibits Golgi membrane fusion. This suggests that it is involved in another cellular function catalysed by p97, the most likely being ubiquitin-dependent events during mitosis. The fact that the binding of p47 and Ufd1/Npl4 is mutually exclusive suggests that these protein complexes act as adapters, directing a basic p97 activity into different cellular pathways.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nploc4RatGolgi organization involved_inIDA PMID:10811609HGNC-UCL 
Nploc4Ratmembrane fusion NOTIDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nploc4RatUFD1-NPL4 complex part_ofIDA PMID:10811609ParkinsonsUK-UCL 
Ufd1RatUFD1-NPL4 complex part_ofIDA PMID:10811609ParkinsonsUK-UCL 
Nploc4RatVCP-NPL4-UFD1 AAA ATPase complex part_ofIDA PMID:10811609ParkinsonsUK-UCL 
Ufd1RatVCP-NPL4-UFD1 AAA ATPase complex part_ofIDA PMID:10811609ParkinsonsUK-UCL 
VcpRatVCP-NPL4-UFD1 AAA ATPase complex part_ofIDA PMID:10811609ParkinsonsUK-UCL 
Nsfl1cRatVCP-NSFL1C complex part_ofIPIUniProtKB:P46462PMID:10811609ParkinsonsUK-UCL 
VcpRatVCP-NSFL1C complex part_ofIPIUniProtKB:O35987PMID:10811609ParkinsonsUK-UCL 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Nploc4  (NPL4 homolog, ubiquitin recognition factor)
Nsfl1c  (NSFL1 cofactor)
Ube4b  (ubiquitination factor E4B)
Ufd1  (ubiquitin recognition factor in ER associated degradation 1)
Vcp  (valosin-containing protein)


Additional Information