RGD Reference Report - Cloning, sequencing, and heterologous expression of rat methionine synthase cDNA. - Rat Genome Database

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Cloning, sequencing, and heterologous expression of rat methionine synthase cDNA.

Authors: Yamada, K  Tobimatsu, T  Toraya, T 
Citation: Yamada K, etal., Biosci Biotechnol Biochem 1998 Nov;62(11):2155-60.
RGD ID: 633492
Pubmed: PMID:9972236   (View Abstract at PubMed)

Methionine synthase catalyzes cobalamin-dependent methyl transfer reaction from 5-methyltetrahydrofolate to homocysteine, forming methionine. Rat methonine synthase cDNA was cloned and analyzed by RT-PCR, 3'- and 5'-RACE techniques. The cDNA consists of a 0.3-kb upstream untranslated region, a 3.8-kb coding region, and a 0.4-kb downstream untranslated region. The open reading frame encoded a polypeptide of 1,253 amino acid residues with a calculated molecular weight of 139,162. This molecular weight was in good agreement with the observed one (143,000) of the purified rat liver enzyme. The deduced amino acid sequence was 53, 92, and 64% identical with those of the Escherichia coli, human, and presumptive Caenorhabditis elegans enzymes, respectively. All the fingerprint sequences, forming parts of the cobalamin- and S-adenosylmethionine-binding sites, were completely conserved in the rat methionine synthase. A high-level expression of catalytically active enzyme in insect cells was done by infection with a baculovirus containing the rat methionine synthase cDNA.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MtrRatmethionine synthase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mtr  (5-methyltetrahydrofolate-homocysteine methyltransferase)


Additional Information