RGD Reference Report - p47 is a cofactor for p97-mediated membrane fusion. - Rat Genome Database

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p47 is a cofactor for p97-mediated membrane fusion.

Authors: Kondo, H  Rabouille, C  Newman, R  Levine, TP  Pappin, D  Freemont, P  Warren, G 
Citation: Kondo H, etal., Nature 1997 Jul 3;388(6637):75-8.
RGD ID: 633466
Pubmed: PMID:9214505   (View Abstract at PubMed)
DOI: DOI:10.1038/40411   (Journal Full-text)

At least two distinct ATPases, NSF and p97, are known to be involved in the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of membrane compartments. The NSF-mediated fusion pathway is the best characterized, many of the components having been identified and their functions analysed. In contrast, none of the accessory proteins for the p97-mediated fusion pathway has been identified. Now we have identified the first such component, a protein of relative molecular mass 47,000 (p47), which forms a tight, stoichiometric complex with cytosolic p97 (one trimer of p47 per hexamer of p97). It is essential for the p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments, a process restricted to animal cells. As a homologue of p47 exists in budding yeast, this indicates that it might also be involved in other membrane fusion reactions catalysed by p97, such as karyogamy.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nsfl1cRatcytosol located_inIDA PMID:9214505ParkinsonsUK-UCL 
VcpRatcytosol located_inIDA PMID:9214505ParkinsonsUK-UCL 
Nsfl1cRatVCP-NSFL1C complex part_ofIPIUniProtKB:P46462PMID:9214505ParkinsonsUK-UCL 
VcpRatVCP-NSFL1C complex part_ofIPIUniProtKB:O35987PMID:9214505ParkinsonsUK-UCL 

Molecular Function
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nsfl1cRatprotein binding enablesIPIUniProtKB:P46462PMID:9214505IntAct 
VcpRatprotein binding enablesIPIUniProtKB:O35987PMID:9214505IntAct 

Objects Annotated

Genes (Rattus norvegicus)
Nsfl1c  (NSFL1 cofactor)
Vcp  (valosin-containing protein)

Additional Information