RGD Reference Report - Identification and characterization of a lysosomal transporter for small neutral amino acids. - Rat Genome Database

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Identification and characterization of a lysosomal transporter for small neutral amino acids.

Authors: Sagne, C  Agulhon, C  Ravassard, P  Darmon, M  Hamon, M  El Mestikawy, S  Gasnier, B  Giros, B 
Citation: Sagne C, etal., Proc Natl Acad Sci U S A 2001 Jun 19;98(13):7206-11.
RGD ID: 633316
Pubmed: (View Article at PubMed) PMID:11390972
DOI: Full-text: DOI:10.1073/pnas.121183498

In eukaryotic cells, lysosomes represent a major site for macromolecule degradation. Hydrolysis products are eventually exported from this acidic organelle into the cytosol through specific transporters. Impairment of this process at either the hydrolysis or the efflux step is responsible of several lysosomal storage diseases. However, most lysosomal transporters, although biochemically characterized, remain unknown at the molecular level. In this study, we report the molecular and functional characterization of a lysosomal amino acid transporter (LYAAT-1), remotely related to a family of H+-coupled plasma membrane and synaptic vesicle amino acid transporters. LYAAT-1 is expressed in most rat tissues, with highest levels in the brain where it is present in neurons. Upon overexpression in COS-7 cells, the recombinant protein mediates the accumulation of neutral amino acids, such as gamma-aminobutyric acid, l-alanine, and l-proline, through an H+/amino acid symport. Confocal microscopy on brain sections revealed that this transporter colocalizes with cathepsin D, an established lysosomal marker. LYAAT-1 thus appears as a lysosomal transporter that actively exports neutral amino acids from lysosomes by chemiosmotic coupling to the H+-ATPase of these organelles. Homology searching in eukaryotic genomes suggests that LYAAT-1 defines a subgroup of lysosomal transporters in the amino acid/auxin permease family.

Annotation

Gene Ontology Annotations    

Biological Process

Cellular Component
lysosome  (IDA)

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Slc36a1  (solute carrier family 36 member 1)

Objects referenced in this article
0 SLC36A1 solute carrier family 36 member 1 All species

Additional Information