RGD Reference Report - Isolation and nucleotide sequence of a cDNA clone encoding rat mitochondrial malate dehydrogenase. - Rat Genome Database

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Isolation and nucleotide sequence of a cDNA clone encoding rat mitochondrial malate dehydrogenase.

Authors: Grant, PM  Tellam, J  May, VL  Strauss, AW 
Citation: Grant PM, etal., Nucleic Acids Res 1986 Aug 11;14(15):6053-66.
RGD ID: 633303
Pubmed: PMID:3755817   (View Abstract at PubMed)
PMCID: PMC311621   (View Article at PubMed Central)

We have determined the complete sequence of the rat mitochondrial malate dehydrogenase (mMDH) precursor derived from nucleotide sequence of the cDNA. A single synthetic oligodeoxynucleotide probe was used to screen a rat atrial cDNA library constructed in lambda gt10. A 1.2 kb full-length cDNA clone provided the first complete amino acid sequence of pre-mMDH. The 1014 nucleotide-long open reading frame encodes the 314 residue long mature mMDH protein and a 24 amino acid NH2-terminal extension which directs mitochondrial import and is cleaved from the precursor after import to generate mature mMDH. The amino acid composition of the transit peptide is polar and basic. The pre-mMDH transit peptide shows marked homology with those of two other enzymes targeted to the rat mitochondrial matrix.



Objects referenced in this article
Gene Mdh2 malate dehydrogenase 2 Rattus norvegicus

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