RGD Reference Report - MIR16, a putative membrane glycerophosphodiester phosphodiesterase, interacts with RGS16. - Rat Genome Database

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MIR16, a putative membrane glycerophosphodiester phosphodiesterase, interacts with RGS16.

Authors: Zheng, B  Chen, D  Farquhar, MG 
Citation: Zheng B, etal., Proc Natl Acad Sci U S A 2000 Apr 11;97(8):3999-4004.
RGD ID: 633189
Pubmed: PMID:10760272   (View Abstract at PubMed)
PMCID: PMC18131   (View Article at PubMed Central)

We have identified the protein MIR16 (for Membrane Interacting protein of RGS16) from a yeast two-hybrid screen by using RGS16 as bait. MIR16 shares strong homology with bacterial glycerophosphodiester phosphodiesterases. It interacts with RGS16 and, more weakly, with several other selected RGS proteins. Analysis of deletion mutants showed that the N-terminal region of the RGS domain in RGS16 is required for its interaction with MIR16. MIR16 is an integral membrane glycoprotein, because it remained associated with membrane fractions after alkaline treatment and because, in some cells, it is sensitive to digestion with endoglycosidase H. By immunofluorescence and immunoelectron microscopy, MIR16 was localized on the plasma membrane in liver and kidney and on intracellular membranes in rat pituitary and cultured pituitary cells. MIR16 represents the only integral membrane protein identified thus far to interact with an RGS domain and, to our knowledge, is the only mammalian glycerophosphodiester phosphodiesterase that has been cloned. The putative enzymatic activity of MIR16 and its interaction with RGS16 suggest that it may play important roles in lipid metabolism and in G protein signaling.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
G protein-coupled receptor signaling pathway  NAS 633189 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gde1  (glycerophosphodiester phosphodiesterase 1)

Objects referenced in this article
Gene Gde1 glycerophosphodiester phosphodiesterase 1 Mus musculus

Additional Information