RGD Reference Report - Kinesin light-chain KLC3 expression in testis is restricted to spermatids. - Rat Genome Database

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Kinesin light-chain KLC3 expression in testis is restricted to spermatids.

Authors: Junco, A  Bhullar, B  Tarnasky, HA  Van der Hoorn, FA 
Citation: Junco A, etal., Biol Reprod 2001 May;64(5):1320-30.
RGD ID: 633112
Pubmed: PMID:11319135   (View Abstract at PubMed)
PMCID: PMC3161965   (View Article at PubMed Central)

Kinesins are tetrameric motor molecules, consisting of two kinesin heavy chains (KHCs) and two kinesin light chains (KLCs) that are involved in transport of cargo along microtubules. The function of the light chain may be in cargo binding and regulation of kinesin activity. In the mouse, two KLC genes, KLC1 and KLC2, had been identified. KLC1 plays a role in neuronal transport, and KLC2 appears to be more widely expressed. We report the cloning from a testicular cDNA expression library of a mammalian light chain, KLC3. The KLC3 gene is located in close proximity to the ERCC2 gene. KLC3 can be classified as a genuine light chain: it interacts in vitro with the KHC, the interaction is mediated by a conserved heptad repeat sequence, and it associates in vitro with microtubules. In mouse and rat testis, KLC3 protein expression is restricted to round and elongating spermatids, and KLC3 is present in sperm tails. In contrast, KLC1 and KLC2 can only be detected before meiosis in testis. Interestingly, the expression profiles of the three known KHCs and KLC3 differ significantly: Kif5a and Kif5b are not expressed after meiosis, and Kif5c is expressed at an extremely low level in spermatids but is not detectable in sperm tails. Our characterization of the KLC3 gene suggests that it carries out a unique and specialized role in spermatids.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
kinesin complex  TAS 633112 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
microtubule motor activity  TAS 633112 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Klc3  (kinesin light chain 3)

Objects referenced in this article
Gene Klc1 kinesin light chain 1 Mus musculus
Gene Klc3 kinesin light chain 3 Mus musculus

Additional Information