RGD Reference Report - Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel. - Rat Genome Database

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Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel.

Authors: Douglass, J  Osborne, PB  Cai, YC  Wilkinson, M  Christie, MJ  Adelman, JP 
Citation: Douglass J, etal., J Immunol 1990 Jun 15;144(12):4841-50.
RGD ID: 633085
Pubmed: PMID:2351830   (View Abstract at PubMed)

Low stringency hybridization screening of a rat genomic DNA library with a previously described cDNA clone encoding a rat voltage-gated potassium channel has resulted in the characterization of a member of the potassium channel family, RGK5. An uninterrupted nucleotide sequence encodes a protein 525 amino acids in length, revealing that the entire coding region resides on a single exon. RGK5 transcripts are present in both mouse thymus and rat brain, as determined by Northern blot analysis. RNA transcribed in vitro from RGK5 genomic DNA directs the expression of functional potassium currents after injection into Xenopus oocytes. The currents are activated by depolarization, being half-activated at -14 mV, and inactivate almost completely during depolarizations of 1 to 2 s. The properties of the currents strongly resemble those of the type n potassium channel present on both immature thymocytes and Th lymphocytes.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcna3Ratpotassium ion transport  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcna3Ratvoltage-gated potassium channel activity  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kcna3  (potassium voltage-gated channel subfamily A member 3)

Objects referenced in this article
Gene Il9 interleukin 9 Rattus norvegicus

Additional Information