RGD Reference Report - Characterization of a cis-Golgi matrix protein, GM130. - Rat Genome Database

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Characterization of a cis-Golgi matrix protein, GM130.

Authors: Nakamura, N  Rabouille, C  Watson, R  Nilsson, T  Hui, N  Slusarewicz, P  Kreis, TE  Warren, G 
Citation: Nakamura N, etal., J Cell Biol 1995 Dec;131(6 Pt 2):1715-26.
RGD ID: 632909
Pubmed: (View Article at PubMed) PMID:8557739

Antisera raised to a detergent- and salt-resistant matrix fraction from rat liver Golgi stacks were used to screen an expression library from rat liver cDNA. A full-length clone was obtained encoding a protein of 130 kD (termed GM130), the COOH-terminal domain of which was highly homologous to a Golgi human auto-antigen, golgin-95 (Fritzler et al., 1993). Biochemical data showed that GM130 is a peripheral cytoplasmic protein that is tightly bound to Golgi membranes and part of a larger oligomeric complex. Predictions from the protein sequence suggest that GM130 is an extended rod-like protein with coiled-coil domains. Immunofluorescence microscopy showed partial overlap with medial- and trans-Golgi markers but almost complete overlap with the cis-Golgi network (CGN) marker, syntaxin5. Immunoelectron microscopy confirmed this location showing that most of the GM130 was located in the CGN and in one or two cisternae on the cis-side of the Golgi stack. GM130 was not re-distributed to the ER in the presence of brefeldin A but maintained its overlap with syntaxin5 and a partial overlap with the ER-Golgi intermediate compartment marker, p53. Together these results suggest that GM130 is part of a cis-Golgi matrix and has a role in maintaining cis-Golgi structure.



Gene Ontology Annotations    

Cellular Component

Objects Annotated

Genes (Rattus norvegicus)
Golga2  (golgin A2)


Additional Information