RGD Reference Report - Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits. - Rat Genome Database

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Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits.

Authors: Chatterton, JE  Awobuluyi, M  Premkumar, LS  Takahashi, H  Talantova, M  Shin, Y  Cui, J  Tu, S  Sevarino, KA  Nakanishi, N  Tong, G  Lipton, SA  Zhang, D 
Citation: Chatterton JE, etal., Nature 2002 Feb 14;415(6873):793-8.
RGD ID: 632842
Pubmed: PMID:11823786   (View Abstract at PubMed)
DOI: DOI:10.1038/nature715   (Journal Full-text)

The N-methyl-D-aspartate subtype of glutamate receptor (NMDAR) serves critical functions in physiological and pathological processes in the central nervous system, including neuronal development, plasticity and neurodegeneration. Conventional heteromeric NMDARs composed of NR1 and NR2A-D subunits require dual agonists, glutamate and glycine, for activation. They are also highly permeable to Ca2+, and exhibit voltage-dependent inhibition by Mg2+. Coexpression of NR3A with NR1 and NR2 subunits modulates NMDAR activity. Here we report the cloning and characterization of the final member of the NMDAR family, NR3B, which shares high sequence homology with NR3A. From in situ and immunocytochemical analyses, NR3B is expressed predominantly in motor neurons, whereas NR3A is more widely distributed. Remarkably, when co-expressed in Xenopus oocytes, NR3A or NR3B co-assembles with NR1 to form excitatory glycine receptors that are unaffected by glutamate or NMDA, and inhibited by D-serine, a co-activator of conventional NMDARs. Moreover, NR1/NR3A or -3B receptors form relatively Ca2+-impermeable cation channels that are resistant to Mg2+, MK-801, memantine and competitive antagonists. In cerebrocortical neurons containing NR3 family members, glycine triggers a burst of firing, and membrane patches manifest glycine-responsive single channels that are suppressible by D-serine. By itself, glycine is normally thought of as an inhibitory neurotransmitter. In contrast, these NR1/NR3A or -3B 'NMDARs' constitute a type of excitatory glycine receptor.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to glycine  IDA 632842 RGD 
ionotropic glutamate receptor signaling pathway  IDA 632842 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
NMDA selective glutamate receptor complex  IDA 632842 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glycine binding  IDA 632842 RGD 
monoatomic cation channel activity  IDA 632842 RGD 
neurotransmitter receptor activity  IDA 632842 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
excitatory synaptic transmission pathway  ISOGrin3b (Rattus norvegicus)632842; 632842 RGD 
excitatory synaptic transmission pathway  IDA 632842 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Grin3b  (glutamate ionotropic receptor NMDA type subunit 3B)

Genes (Mus musculus)
Grin3b  (glutamate receptor, ionotropic, NMDA3B)

Genes (Homo sapiens)
GRIN3B  (glutamate ionotropic receptor NMDA type subunit 3B)

Additional Information