RGD Reference Report - Cloning and expression of a rat brain GABA transporter. - Rat Genome Database

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Cloning and expression of a rat brain GABA transporter.

Authors: Guastella, J  Nelson, N  Nelson, H  Czyzyk, L  Keynan, S  Miedel, MC  Davidson, N  Lester, HA  Kanner, BI 
Citation: Guastella J, etal., Science 1990 Sep 14;249(4974):1303-6.
RGD ID: 632739
Pubmed: (View Article at PubMed) PMID:1975955

A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter gamma-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in Xenopus oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated [3H]GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium-and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules.

Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Slc6a1  (solute carrier family 6 member 1)

Additional Information