RGD Reference Report - Isolation of rat fibrillin-1 cDNA and its relevance in metanephric development. - Rat Genome Database

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Isolation of rat fibrillin-1 cDNA and its relevance in metanephric development.

Authors: Kanwar, YS  Ota, K  Yang, Q  Kumar, A  Wada, J  Kashihara, N  Peterson, DR 
Citation: Kanwar YS, etal., Am J Physiol 1998 Nov;275(5 Pt 2):F710-23.
RGD ID: 632672
Pubmed: PMID:9815129   (View Abstract at PubMed)

The role of fibrillin-1 in metanephrogenesis was investigated. Fibrillin-1 cDNA was isolated from the rat kidney cDNA library and sequenced, and its spatiotemporal expression was studied. It had approximately 88% homology with human fibrillin-1 and had Ca2+ binding epidermal growth factor-like domains, transforming growth factor-beta binding protein motifs, and an RGD binding site. Northern blot analysis revealed an approximately 10-kb transcript, and fibrillin-1 expression was developmentally regulated. In situ hybridization and immunofluorescence studies indicated that at day 15 of gestation, fibrillin-1 is expressed in the metanephric mesenchyme. At day 18, its expression was confined to nascent blood vessels and glomeruli, and it increased in the newborn and neonatal kidneys. Immunoprecipitation revealed an approximately 300-kDa band by SDS-PAGE. Treatment with fibrillin-1 antisense oligodeoxynucleotide induced marked dysmorphogenesis of the embryonic metanephroi. Concomitantly, the fibrillin-1 mRNA, antibody reactivity in the metanephroi, and fibrillin-1-specific radioincorporation were reduced. These data indicate that, like alphavbeta3 integrin, a known morphogen and a putative receptor of fibrillin-1, the fibrillin-1 modulates events related to early organogenesis and possibly also the vascularization of the rat kidney.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
kidney development  IEP 632672 RGD 
kidney development  IMP 632672 RGD 
metanephros development  IMP 632672 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fbn1  (fibrillin 1)


Additional Information