RGD Reference Report - The nucleotide and deduced amino acid sequence of a rat cysteine string protein. - Rat Genome Database

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The nucleotide and deduced amino acid sequence of a rat cysteine string protein.

Authors: Mastrogiacomo, A  Gundersen, CB 
Citation: Mastrogiacomo A and Gundersen CB, Brain Res Mol Brain Res 1995 Jan;28(1):12-8.
RGD ID: 632551
Pubmed: PMID:7535880   (View Abstract at PubMed)

Cysteine string proteins are novel, heavily lipidated components of synaptic vesicles. They have previously been studied in Drosophila (insect) and Torpedo (fish). To facilitate further investigation of the structure and function of these proteins in mammals, we isolated and sequenced the cDNA and conducted an initial characterization of a rat cysteine string protein. Nucleotide sequencing reveals that this rat protein is highly homologous to the insect and fish cysteine string proteins. At the amino acid level, the fish and rat proteins are 82% identical. The rat cysteine string protein is encoded by an approximately 5 kb mRNA that is ubiquitously expressed in rat brain. Using antibodies that cross-react with the rat protein, we find that the rat cysteine string protein is predominantly associated with nerve endings and synaptic vesicles. Moreover, like its Torpedo (fish) counterpart, it is extensively fatty acylated. It will be of considerable interest to ascertain the functional correlates of these cross-species similarities of cysteine string proteins.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
synaptic vesicle membrane is_active_inIDA 632551PMID:7535880SynGO 
synaptic vesicle membrane is_active_inIEP 632551PMID:7535880SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Dnajc5  (DnaJ heat shock protein family (Hsp40) member C5)


Additional Information