RGD Reference Report - Expression of the alpha1F calcium channel subunit by photoreceptors in the rat retina. - Rat Genome Database

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Expression of the alpha1F calcium channel subunit by photoreceptors in the rat retina.

Authors: Morgans, CW  Gaughwin, P  Maleszka, R 
Citation: Morgans CW, etal., Mol Vis 2001 Aug 22;7:202-9.
RGD ID: 632484
Pubmed: PMID:11526344   (View Abstract at PubMed)

PURPOSE: The CACNA1F gene encodes a voltage-gated calcium channel alpha1 subunit, alpha1F, which is expressed in the human retina. Mutations in this gene cause incomplete X-linked congenital stationary night blindness (CSNB2). The aim of this study was to obtain the sequence of the rat alpha1F cDNA and localize the encoded polypeptide in the rat retina. METHODS: The full-length rat alpha1F sequence was compiled from sequencing of overlapping alpha1F PCR fragments amplified from rat retinal cDNA. Antiserum was raised against a human alpha1F peptide. It was found that the human alpha1F peptide used to generate the antiserum was conserved at only 11 out of 19 residues in the cloned rat sequence. Therefore, antibodies were affinity purified against either the human alpha1F peptide or the equivalent rat peptide and used for immunofluorescent staining of rat retina sections. RESULTS: The rat alpha1F amino acid sequence was found to be 91% and 95% identical to the human and mouse alpha1F sequences, respectively. Antibodies affinity purified against the human alpha1F peptide stained both the outer nuclear layer (ONL) and outer plexiform layer of rat retina sections. In contrast, staining with antibodies affinity purified against the corresponding rat alpha1F peptide was restricted to the ONL. CONCLUSIONS: The rat alpha1F amino acid sequence is highly homologous to the human and mouse sequences. The immunohistochemical results indicate the existence of distinct alpha1F isoforms or alpha1F-like channels, which are differentially distributed in the cell bodies and synaptic terminals of photoreceptors in the rat retina.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
neurotransmitter secretion  NAS 632484 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
neuronal cell body  IDA 632484 RGD 
photoreceptor outer segment  IDA 632484 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
high voltage-gated calcium channel activity  ICGO:0007601632484 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cacna1f  (calcium voltage-gated channel subunit alpha1 F)

Objects referenced in this article
Gene Cacna1f calcium channel, voltage-dependent, alpha 1F subunit Mus musculus

Additional Information