RGD Reference Report - Cloning of three novel neuronal Cdk5 activator binding proteins. - Rat Genome Database

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Cloning of three novel neuronal Cdk5 activator binding proteins.

Authors: Ching, YP  Qi, Z  Wang, JH 
Citation: Ching YP, etal., Gene 2000 Jan 25;242(1-2):285-94.
RGD ID: 632478
Pubmed: PMID:10721722   (View Abstract at PubMed)

Neuronal Cdc2-like kinase (Nclk) is involved in the regulation of neuronal differentiation and neuro-cytoskeleton dynamics. The active kinase consists of a catalytic subunit, Cdk5, and a 25 kDa activator protein (p25nck5a) derived from a 35 kDa neuronal-specific protein (p35nck5a). As an extension of our previous study (Qi, Z., Tang, D., Zhu, X., Fujita, D.J., Wang, J.H., 1998. Association of neurofilament proteins with neuronal Cdk5 activator. J. Biol. Chem. 270, 2329-2335), which showed that neurofilament is one of the p35nck5a-associated proteins, we now report the isolation of three other novel p35nck5a-associated proteins using the yeast two-hybrid screen. The full-length forms of these three novel proteins, designated C42, C48 and C53, have a molecular mass of 66, 24, and 57 kDa, respectively. Northern analysis indicates that these novel proteins are widely expressed in human tissues, including the heart, brain, skeletal muscle, placenta, lung, liver, kidney and pancreas. The bacterially expressed glutathione S-transferase (GST)-fusion forms of these three proteins were able to co-precipitate p35nck5a complexed with Cdk5 from insect cell lysate. Among these three proteins, only C48 and C53 can be phosphorylated by Nclk, suggesting that they may be the substrates of Nclk. Sequence homology searches have suggested that the C48 protein is marginally related to restin protein, whereas the C42 protein has homologues of unknown function in Caenorhabditis elegans and Arabidopsis thaliana.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein-containing complex binding  IDA 632478; 632478 RGD 
protein-containing complex binding  IDA 632478Cdk5 and Cdk5r1RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cdk5rap1  (CDK5 regulatory subunit associated protein 1)
Cdk5rap2  (CDK5 regulatory subunit associated protein 2)
Cdk5rap3  (CDK5 regulatory subunit associated protein 3)

Objects referenced in this article
Gene CDK5RAP2 CDK5 regulatory subunit associated protein 2 Homo sapiens
Gene CDK5RAP3 CDK5 regulatory subunit associated protein 3 Homo sapiens

Additional Information