RGD Reference Report - Ligand-specific control of src-suppressed C kinase substrate gene expression. - Rat Genome Database

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Ligand-specific control of src-suppressed C kinase substrate gene expression.

Authors: Coats, SR  Pabon-Pena, LM  Covington, JW  Vaughan, DE 
Citation: Coats SR, etal., Biochem Biophys Res Commun 2002 Oct 11;297(5):1112-20.
RGD ID: 631986
Pubmed: PMID:12372401   (View Abstract at PubMed)

The src-suppressed C-kinase substrate, SSeCKS, is now recognized as a key regulator of cell signaling and cytoskeletal dynamics. However, few ligands that control SSeCKS expression have been identified. We report that platelet-derived growth factor-BB (PDGF-BB), lysophosphatidic acid (LPA), and eicosapentaenoic acid (EPA) potently modulate SSeCKS gene expression in cultured smooth muscle (RASM) cells relative to other bioactive ligands tested. In addition, EPA-dependent regulation of SSeCKS expression correlates with distinct changes in cell morphology and adhesion in RASM cells. Independent evidence that ligand-specific control of SSeCKS expression links to the regulation of cell adhesion and morphology was obtained using ras-transformed fibroblasts, KNRK. Sodium butyrate (NaB) upregulates SSeCKS mRNA and protein expression corresponding to increased cell-spreading and adhesion. In addition, ectopic expression of recombinant SSeCKS recapitulates attributes of NaB-induced morphogenesis in KNRK cells. The data provide novel evidence that SSeCKS functions in PDGF-BB-, LPA-, EPA-, and NaB-mediated cell signaling.

Objects referenced in this article
Gene Akap12 A-kinase anchoring protein 12 Rattus norvegicus

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