RGD Reference Report - A rat brain cDNA encodes enzymatically active GABA transaminase and provides a molecular probe for GABA-catabolizing cells. - Rat Genome Database

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A rat brain cDNA encodes enzymatically active GABA transaminase and provides a molecular probe for GABA-catabolizing cells.

Authors: Medina-Kauwe, LK  Tillakaratne, NJ  Wu, JY  Tobin, AJ 
Citation: Medina-Kauwe LK, etal., J Neurochem 1994 Apr;62(4):1267-75.
RGD ID: 631979
Pubmed: PMID:8133261   (View Abstract at PubMed)

cDNAs encoding gamma-aminobutyric acid aminotransferase (GABA-T) were isolated from a lambda ZAP rat hippocampal cDNA expression library by two independent cloning methods, immunological screening with an antimouse GABA-T antibody and plaque hybridization with a GABA-T cDNA probe derived by polymerase chain reaction. We have produced enzymatically active GABA-T from a rat brain cDNA containing the full-length GABA-T coding region. Our rat brain GABA-T cDNAs hybridize to mRNAs in brain and peripheral tissues, including liver, kidney, and testis. We have also detected GABA-T mRNA in GABAergic cells of rat cerebellar cortex by in situ hybridization. Our rat brain GABA-T probe hybridizes to Purkinje, basket, stellate, and Golgi II cells, the same GABAergic neurons previously shown to contain glutamate decarboxylase GAD65 and GAD67.

Objects referenced in this article
Gene Abat 4-aminobutyrate aminotransferase Rattus norvegicus

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