RGD Reference Report - Molecular and immunological characterization of ADP-ribosylarginine hydrolases. - Rat Genome Database

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Molecular and immunological characterization of ADP-ribosylarginine hydrolases.

Authors: Moss, J  Stanley, SJ  Nightingale, MS  Murtagh JJ, JR  Monaco, L  Mishima, K  Chen, HC  Williamson, KC  Tsai, SC 
Citation: Moss J, etal., J Biol Chem 1992 May 25;267(15):10481-8.
RGD ID: 631707
Pubmed: PMID:1375222   (View Abstract at PubMed)

Mono-ADP-ribosylation is a reversible modification of proteins with NAD:arginine ADP-ribosyltransferases and ADP-ribosylarginine hydrolases catalyzing the forward and reverse reactions, respectively. Hydrolase activities were present in a variety of animal species, with the highest specific activities found in rat and mouse brain, spleen, and testis. Rat and mouse hydrolases were dithiothreitol- and Mg(2+)-dependent, whereas the bovine and guinea pig enzymes were dithiothreitol-independent. A rat brain hydrolase was purified approximately 20,000-fold and represented the major approximately 39-kDa protein on denaturing gels. Immunoaffinity-purified rabbit polyclonal antibodies reacted with 39-kDa proteins from turkey erythrocytes and rat, mouse, and calf brains. A rat brain cDNA library was screened using oligonucleotide and polymerase chain reaction-generated cDNA probes. Inserts from two overlapping clones yielded a composite sequence that included a 1086-base pair open reading frame, which contained amino acid sequences found in the purified hydrolase. A hydrolase fusion protein, synthesized in Escherichia coli, reacted with anti-39-kDa polyclonal antibodies and exhibited Mg(2+)- and dithiothreitol-dependent hydrolase activity. A coding region cDNA hybridized readily to a 1.7-kilobase band in rat and mouse poly(A)+ RNA, but poorly to bovine, chicken, rabbit, and human poly(A)+ RNA. The immunological and molecular biological data are consistent with partial conservation of hydrolase structure across animal species.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein modification process involved_inIMP 631707PMID:1375222UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ADP-ribosylarginine hydrolase activity enablesIMP 631707PMID:1375222UniProt 
ADP-ribosylarginine hydrolase activity  IDA 631707 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Adprh  (ADP-ribosylarginine hydrolase)


Additional Information