RGD Reference Report - Substrate-dependent regulation of MAO-A in rat mesangial cells: involvement of dopamine D2-like receptors. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Substrate-dependent regulation of MAO-A in rat mesangial cells: involvement of dopamine D2-like receptors.

Authors: Pizzinat, N  Marchal-Victorion, S  Maurel, A  Ordener, C  Bompart, G  Parini, A 
Citation: Pizzinat N, etal., Am J Physiol Renal Physiol 2003 Jan;284(1):F167-74.
RGD ID: 629546
Pubmed: (View Article at PubMed) PMID:12388421
DOI: Full-text: DOI:10.1152/ajprenal.00113.2002

In the present study, we investigated the existence of a back-regulation of the catecholamine-degrading enzyme monoamine oxidase (MAO)-A by dopamine in rat renal cells. In proximal tubule cells, MAO-A expression was not modified after dopamine receptor stimulation. In contrast, in mesangial cells, enzyme assay and Western blots showed that MAO activity and protein increased by approximately 80% after 48-h incubation with the D(2)-like receptor agonist bromocriptine and quinpirole but not with the D(1)-like receptor agonist SKF-38393. This effect was prevented by the D(2)-receptor antagonist sulpiride and domperidone. The increase in MAO-A protein was preceded by an augmentation of MAO-A mRNA that was prevented by the transcriptional inhibitor actinomycin D. Bromocriptine effect was mimicked by the PKA inhibitor H89 and inhibited by the PKA activator 8-bromo-cAMP. These results show for the first time the existence of a dopamine-dependent MAO-A regulation involving D(2)-like receptors, inhibition of the cAMP-PKA pathway, and an ex novo enzyme synthesis.

Annotation

Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Drd2  (dopamine receptor D2)
Maoa  (monoamine oxidase A)


Additional Information