RGD Reference Report - Substrate-dependent regulation of MAO-A in rat mesangial cells: involvement of dopamine D2-like receptors. - Rat Genome Database

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Substrate-dependent regulation of MAO-A in rat mesangial cells: involvement of dopamine D2-like receptors.

Authors: Pizzinat, N  Marchal-Victorion, S  Maurel, A  Ordener, C  Bompart, G  Parini, A 
Citation: Pizzinat N, etal., Am J Physiol Renal Physiol 2003 Jan;284(1):F167-74.
RGD ID: 629546
Pubmed: PMID:12388421   (View Abstract at PubMed)
DOI: DOI:10.1152/ajprenal.00113.2002   (Journal Full-text)

In the present study, we investigated the existence of a back-regulation of the catecholamine-degrading enzyme monoamine oxidase (MAO)-A by dopamine in rat renal cells. In proximal tubule cells, MAO-A expression was not modified after dopamine receptor stimulation. In contrast, in mesangial cells, enzyme assay and Western blots showed that MAO activity and protein increased by approximately 80% after 48-h incubation with the D(2)-like receptor agonist bromocriptine and quinpirole but not with the D(1)-like receptor agonist SKF-38393. This effect was prevented by the D(2)-receptor antagonist sulpiride and domperidone. The increase in MAO-A protein was preceded by an augmentation of MAO-A mRNA that was prevented by the transcriptional inhibitor actinomycin D. Bromocriptine effect was mimicked by the PKA inhibitor H89 and inhibited by the PKA activator 8-bromo-cAMP. These results show for the first time the existence of a dopamine-dependent MAO-A regulation involving D(2)-like receptors, inhibition of the cAMP-PKA pathway, and an ex novo enzyme synthesis.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
catecholamine catabolic process  NAS 629546 RGD 
positive regulation of transcription by RNA polymerase II  IDA 629546 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
primary amine oxidase activity  IDA 629546 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Drd2  (dopamine receptor D2)
Maoa  (monoamine oxidase A)


Additional Information