RGD Reference Report - Vasopressin increases intracellular NO concentration via Ca(2+) signaling in inner medullary collecting duct. - Rat Genome Database

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Vasopressin increases intracellular NO concentration via Ca(2+) signaling in inner medullary collecting duct.

Authors: Mori, T  Dickhout, JG  Cowley AW, JR 
Citation: Mori T, etal., Hypertension 2002 Feb;39(2 Pt 2):465-9.
RGD ID: 628560
Pubmed: PMID:11882591   (View Abstract at PubMed)

The present study was designed to determine whether arginine vasopressin (AVP) stimulates NO production in the epithelial collecting duct cells of the inner medulla (IMCDs) and if this is mediated through Ca(2+) signaling. Thin tissue layers containing IMCDs were dissected from Sprague-Dawley rats. Intracellular Ca(2+) concentration ([Ca(2+)](i)) and NO production were measured in IMCDs by a fluorescence imaging system with the use of fura 2-AM and the cell-permeable form of the NO-sensitive dye 4,5-diaminofluorescein (DAF-2), respectively. AVP (100 nmol/L) produced a rapid peak increase in [Ca(2+)](i) of 320 +/- 70 nmol/L within a few seconds and a sustained increase of 120 +/- 62 nmol/L. The peak increase in [Ca(2+)](i) was followed by a significant increase of NO production (34 +/- 7 U). This was similar to that produced by 20 micromol/L of the NO donor DETA-NONOate (42 +/- 11 U). The NO scavenger carboxy-PTIO (100 micromol/L) or depletion of [Ca(2+)](i) by preincubation with 5 micromol/L of the Ca(2+)-ATPase inhibitor thapsigargin in Ca(2+)-free buffer abolished the NO response to AVP. We conclude that AVP mobilizes Ca(2+) to produce NO in IMCDs.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Avpr2Ratpeptide hormone binding  TAS  RGD 
AvpRatV2 vasopressin receptor binding  TAS  RGD 
Avpr2Ratvasopressin receptor activity  TAS  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AVPR2Humanvasopressin signaling pathway   ISOAvpr2 (Rattus norvegicus) RGD 
Avpr2Ratvasopressin signaling pathway   TAS  RGD 
Avpr2Mousevasopressin signaling pathway   ISOAvpr2 (Rattus norvegicus) RGD 
Objects Annotated

Genes (Rattus norvegicus)
Avp  (arginine vasopressin)
Avpr2  (arginine vasopressin receptor 2)

Genes (Mus musculus)
Avpr2  (arginine vasopressin receptor 2)

Genes (Homo sapiens)
AVPR2  (arginine vasopressin receptor 2)


Additional Information