RGD Reference Report - Expression and canalicular localization of two isoforms of the ClC-3 chloride channel from rat hepatocytes. - Rat Genome Database

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Expression and canalicular localization of two isoforms of the ClC-3 chloride channel from rat hepatocytes.

Authors: Shimada, K  Li, X  Xu, G  Nowak, DE  Showalter, LA  Weinman, SA 
Citation: Shimada K, etal., Am J Physiol Gastrointest Liver Physiol 2000 Aug;279(2):G268-76.
RGD ID: 628537
Pubmed: PMID:10915634   (View Abstract at PubMed)

The molecular identities of functional chloride channels in hepatocytes are largely unknown. We examined the ClC-3 chloride channel in rat hepatocytes and found that mRNA for two different isoforms is present. A short form is identical to the previously reported sequence for rat ClC-3, and a long form contains a 176-bp insertion immediately upstream of the translation initiation site. This predicts a 58-amino acid NH(2) terminal insertion. Both long and short form mRNA was expressed in diverse tissues of the rat. Transient transfection of the long form in CHO-K1 cells resulted in currents with an I(-) > B(-) > Cl(-) selectivity sequence, outward rectification, and inactivation at positive voltages. Short form currents had identical ionic selectivity but displayed a more extreme outward rectification and showed no voltage-dependent inactivation. Immunofluorescence and immunoblots localized native ClC-3 preferentially but not exclusively to the canalicular membrane. We have therefore identified a new isoform of rat ClC-3 and shown that expression of both isoforms produces functional channels. In hepatocytes, ClC-3 is located in association with the canalicular membrane.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Clcn3Ratchloride transport  IDA  RGD 
Clcn3Ratmonoatomic ion transport  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Clcn3Ratplasma membrane located_inIDA PMID:10915634UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Clcn3Ratchloride channel activity  IDA  RGD 
Clcn3Ratmonoatomic ion channel activity  IDA  RGD 
Clcn3Ratvoltage-gated chloride channel activity enablesIDA PMID:10915634UniProt 
Clcn3Ratvoltage-gated chloride channel activity  IDA  RGD 
Clcn3Ratvoltage-gated monoatomic ion channel activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Clcn3  (chloride voltage-gated channel 3)

Objects referenced in this article
Gene Clcn5 chloride voltage-gated channel 5 Rattus norvegicus

Additional Information