RGD Reference Report - Identification and characterization of a synaptojanin 2 splice isoform predominantly expressed in nerve terminals. - Rat Genome Database

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Identification and characterization of a synaptojanin 2 splice isoform predominantly expressed in nerve terminals.

Authors: Nemoto, Y  Wenk, MR  Watanabe, M  Daniell, L  Murakami, T  Ringstad, N  Yamada, H  Takei, K  De Camilli, P 
Citation: Nemoto Y, etal., J Biol Chem 2001 Nov 2;276(44):41133-42.
RGD ID: 628465
Pubmed: PMID:11498538   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M106404200   (Journal Full-text)

We have previously identified synaptojanin 1, a phosphoinositide phosphatase predominantly expressed in the nervous system, and synaptojanin 2, a broadly expressed isoform. Synaptojanin 1 is concentrated in nerve terminals, where it has been implicated in synaptic vesicle recycling and actin function. Synaptojanin 2A is targeted to mitochondria via a PDZ domain-mediated interaction. We have now characterized an alternatively spliced form of synaptojanin 2 that shares several properties with synaptojanin 1. This isoform, synaptojanin 2B, undergoes further alternative splicing to generate synaptojanin 2B1 and 2B2. Both amphiphysin and endophilin, two partners synaptojanin 1, bind synaptojanin 2B2, whereas only amphiphysin binds synaptojanin 2B1. Sequence similar to the endophilin-binding site in synaptojanin 1 is present only in synaptojanin 2B2, and this sequence was capable of affinity purifying endophilin from rat brain. The Sac1 domain of synaptojanin 2 exhibited phosphoinositide phosphatase activity very similar to that of the Sac1 domain of synaptojanin 1. Site-directed mutagenesis further illustrated its functional similarity to the catalytic domain of Sac1 proteins. Antibodies raised against the synaptojanin 2B-specific carboxyl-terminal region identified a 160-kDa protein in brain and testis. Immunofluorescence showed that synaptojanin 2B is localized at nerve terminals in brain and at the spermatid manchette in testis. Active Rac1 GTPase affects the intracellular localization of synaptojanin 2, but not of synaptojanin 1. These results suggest that synaptojanin 2B has a partially overlapping function with synaptojanin 1 in nerve terminals, with additional roles in neurons and other cells including spermatids.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Synj2Ratphosphatidylinositol dephosphorylation  IDA hydrolyzes phosphate from PI3P, PI4P, and PI3,5P2, but not PI4,5P2RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Synj2Rataxon terminus  IDA  RGD 
Synj2_v2Rataxon terminus  IDA  RGD 
Synj2_v3Rataxon terminus  IDA  RGD 
Synj2Ratcytoplasmic microtubule  IDA in the microtubular manchette formation surrounding spermatid nucleiRGD 
Synj2_v2Ratcytoplasmic microtubule  IDA  RGD 
Synj2_v3Ratcytoplasmic microtubule  IDA  RGD 
Synj2Ratpresynapse is_active_inIDA PMID:11498538SynGO 
Synj2Ratruffle membrane  IDA when coexpressed with constitutively GTP-bound Rac1RGD 
Synj2_v1Ratruffle membrane  IDA when coexpressed with constitutively GTP-bound Rac1RGD 
Synj2_v2Ratruffle membrane  IDA when coexpressed with constitutively GTP-bound Rac1RGD 
Synj2_v3Ratruffle membrane  IDA when coexpressed with constitutively GTP-bound Rac1RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Synj2RatPDZ domain binding NOTIPIRGD:69400neither Synj2_v1 nor Synj2_v2 bind Synj2bpRGD 
Synj2_v1RatPDZ domain binding  IPIRGD:69400 RGD 
Synj2_v2RatPDZ domain binding NOTIPIRGD:69400neither Synj2_v1 nor Synj2_v2 bind Synj2bpRGD 
AmphRatphosphatase binding  IPIRGD:69434protein C-terminus bindingRGD 
Synj2Ratphosphatidylinositol monophosphate phosphatase activity  IDA hydrolyzes phosphate from PI3P, PI4P, and PI3,5P2, but not PI4,5P2RGD 
AmphRatprotein binding  IPIRGD:728884protein C-terminus bindingRGD 
AmphRatprotein binding  IPIRGD:728906protein C-terminus bindingRGD 
AmphRatprotein binding  IPIRGD:728898protein C-terminus bindingRGD 
Sh3gl1Ratprotein binding  IPIRGD:728898 RGD 
Sh3gl2Ratprotein binding  IPIRGD:728898 RGD 
Synj2_v3Ratprotein binding  IPIRGD:620276 RGD 
Synj2_v3Ratprotein binding  IPIRGD:708456 RGD 
Synj2bpRatprotein binding NOTIPIRGD:728906protein C-terminus bindingRGD 
Synj1RatSH3 domain binding  IPIRGD:620274also binds SH3 domain of endophilinsRGD 
Synj2RatSH3 domain binding  IPIRGD:620274also binds SH3 domain of endophilinsRGD 
Synj2_v1RatSH3 domain binding NOTIPIRGD:620274also binds SH3 domain of endophilinsRGD 
Synj2_v3RatSH3 domain binding  IPIRGD:620274 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Amph  (amphiphysin)
Sh3gl1  (SH3 domain containing GRB2 like 1, endophilin A2)
Sh3gl2  (SH3 domain containing GRB2 like 2, endophilin A1)
Synj1  (synaptojanin 1)
Synj2  (synaptojanin 2)
Synj2_v1  (synaptojanin 2, variant 1)
Synj2_v2  (synaptojanin 2, variant 2)
Synj2_v3  (synaptojanin 2, variant 3)
Synj2bp  (synaptojanin 2 binding protein)

Objects referenced in this article
Gene Kcnip2_v1 potassium voltage-gated channel interacting protein 2, variant 1 Rattus norvegicus
Gene Kcnip2_v2 potassium voltage-gated channel interacting protein 2, variant 2 Rattus norvegicus
Gene Sh3gl3 SH3 domain containing GRB2 like 3, endophilin A3 Rattus norvegicus

Additional Information