RGD Reference Report - A third vesicular glutamate transporter expressed by cholinergic and serotoninergic neurons. - Rat Genome Database

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A third vesicular glutamate transporter expressed by cholinergic and serotoninergic neurons.

Authors: Gras, C  Herzog, E  Bellenchi, GC  Bernard, V  Ravassard, P  Pohl, M  Gasnier, B  Giros, B  El Mestikawy, S 
Citation: Gras C, etal., J Neurosci 2002 Jul 1;22(13):5442-51.
RGD ID: 628464
Pubmed: PMID:12097496   (View Abstract at PubMed)
PMCID: PMC6758212   (View Article at PubMed Central)

Two proteins previously known as Na(+)-dependent phosphate transporters have been identified recently as vesicular glutamate transporters (VGLUT1 and VGLUT2). Together, VGLUT1 and VGLUT2 are operating at most central glutamatergic synapses. In this study, we characterized a third vesicular glutamate transporter (VGLUT3), highly homologous to VGLUT1 and VGLUT2. Vesicles isolated from endocrine cells expressing recombinant VGLUT3 accumulated l-glutamate with bioenergetic and pharmacological characteristics similar, but not identical, to those displayed by the type-1 and type-2 isoforms. Interestingly, the distribution of VGLUT3 mRNA was restricted to a small number of neurons scattered in the striatum, hippocampus, cerebral cortex, and raphe nuclei, in contrast to VGLUT1 and VGLUT2 transcripts, which are massively expressed in cortical and deep structures of the brain, respectively. At the ultrastructural level, VGLUT3 immunoreactivity was concentrated over synaptic vesicle clusters present in nerve endings forming asymmetrical as well as symmetrical synapses. Finally, VGLUT3-positive neurons of the striatum and raphe nuclei were shown to coexpress acetylcholine and serotonin transporters, respectively. Our study reveals a novel class of glutamatergic nerve terminals and suggests that cholinergic striatal interneurons and serotoninergic neurons from the brainstem may store and release glutamate.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc17a8RatL-glutamate import involved_inIMP PMID:12097496UniProt 
Slc17a8RatL-glutamate transmembrane transport involved_inIMP PMID:12097496UniProt 

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc17a8RatL-glutamate uniporter activity enablesIDA PMID:12097496UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc17a8  (solute carrier family 17 member 8)

Additional Information