RGD Reference Report - IL-10 receptor and coreceptor expression in quiescent and activated hepatic stellate cells. - Rat Genome Database

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IL-10 receptor and coreceptor expression in quiescent and activated hepatic stellate cells.

Authors: Mathurin, P  Xiong, S  Kharbanda, KK  Veal, N  Miyahara, T  Motomura, K  Rippe, RA  Bachem, MG  Tsukamoto, H 
Citation: Mathurin P, etal., Am J Physiol Gastrointest Liver Physiol 2002 Jun;282(6):G981-90.
RGD ID: 625762
Pubmed: PMID:12016123   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpgi.00293.2001   (Journal Full-text)

Interleukin (IL)-10 expression is induced in activated hepatic stellate cells (HSC) in vitro and in vivo. We analyzed expression of IL-10 receptor (IL-10R) and coreceptor cytokine receptor family (CRF2-4) in HSC. We aimed to clone and sequence partial cDNA for rat IL-10R and CRF2-4, determine their expression in activated rat HSC in vivo and in vitro, and examine the biological responsiveness of HSC to exogenous IL-10. PCR cloning and sequencing of partial rat IL-10R and CRF2-4 cDNAs revealed 86% homology with corresponding mouse sequences. In hepatic macrophages, Northern blot with cloned IL-10R cDNA detected an expected 3.5-kb transcript, and IL-10R and CRF2-4 mRNAs showed steady constitutive expression after in vitro lipopolysaccharide treatment or cholestatic liver injury. IL-10R mRNA expression, as confirmed by immunohistochemistry, was induced 20.1- and 8.6-fold in HSC from cholestatic livers and 7-day culture-activated HSC, respectively but CRF2-4 mRNA levels were unchanged. Under serum-free conditions, IL-10 had minimal effects on collagen production but reduced DNA synthesis, matrix metalloprotease-2 mRNA levels, and activity in HSC. With serum, IL-10 inhibited both collagen production and DNA synthesis but had no effect on procollagen-alpha(1)(I) mRNA levels. This shows concomitant induction of IL-10R but not CRF2-4 to that of IL-10 by activated HSC in vitro and in vivo and associated acquisition of the responsiveness to IL-10, entailing complex effects on HSC.

Objects referenced in this article
Gene Il10ra interleukin 10 receptor subunit alpha Rattus norvegicus
Gene Nos2 nitric oxide synthase 2 Rattus norvegicus

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