RGD Reference Report - Attenuation of experimental allergic encephalomyelitis in complement component 6-deficient rats is associated with reduced complement C9 deposition, P-selectin expression, and cellular infiltrate in spinal cords.

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Attenuation of experimental allergic encephalomyelitis in complement component 6-deficient rats is associated with reduced complement C9 deposition, P-selectin expression, and cellular infiltrate in spinal cords.
Authors:	Tran, GT Hodgkinson, SJ Carter, N Killingsworth, M Spicer, ST Hall, BM
Citation:	Tran GT, etal., J Immunol 2002 May 1;168(9):4293-300.
RGD ID:	625607
Pubmed:	PMID:11970970 (View Abstract at PubMed)
The role of Ab deposition and complement activation, especially the membrane attack complex (MAC), in the mediation of injury in experimental allergic encephalomyelitis (EAE) is not resolved. The course of active EAE in normal PVG rats was compared with that in PVG rats deficient in the C6 component of complement (PVG/C6(-)) that are unable to form MAC. Following immunization with myelin basic protein, PVG/C6(-) rats developed significantly milder EAE than PVG/C rats. The anti-myelin basic protein response was similar in both strains, as was deposition of C3 in spinal cord. C9 was detected in PVG/C rats but not in PVG/C6(-), consistent with their lack of C6 and inability to form MAC. In PVG/C6(-) rats, the T cell and macrophage infiltrate in the spinal cord was also significantly less than in normal PVG/C rats. There was also reduced expression of P-selectin on endothelial cells, which may have contributed to the reduced cellular infiltrate by limiting migration from the circulation. Assay of cytokine mRNA by RT-PCR in the spinal cords showed no differences in the profile of Th1 or Th2 cytokines between PVG/C and PVG/C6(-) rats. PVG/C rats also had a greater increase in peripheral blood white blood cell, neutrophil, and basophil counts than was observed in the PVG/C6(-). These findings suggest that the MAC may have a role in the pathogenesis of EAE, not only by Ig-activated MAC injury but also via induction of P-selectin on vascular endothelium to promote infiltration of T cells and macrophages into the spinal cord.

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
C6	Rat	Experimental Autoimmune Encephalomyelitis	severity	IAGP			RGD
C6	Human	Experimental Autoimmune Encephalomyelitis	severity	ISO	C6 (Rattus norvegicus)		RGD
C6	Mouse	Experimental Autoimmune Encephalomyelitis	severity	ISO	C6 (Rattus norvegicus)		RGD

Objects Annotated

Genes (Rattus norvegicus)

C6 (complement C6)

Genes (Mus musculus)

C6 (complement component 6)

Genes (Homo sapiens)

C6 (complement C6)

Objects referenced in this article

Strain	PVG	PVG	Rattus norvegicus

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Attenuation of experimental allergic encephalomyelitis in complement component 6-deficient rats is associated with reduced complement C9 deposition, P-selectin expression, and cellular infiltrate in spinal cords.