RGD Reference Report - Functional characterization of two splice variants of rat bad and their interaction with Bcl-w in sympathetic neurons.PG - 97-106 - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Functional characterization of two splice variants of rat bad and their interaction with Bcl-w in sympathetic neurons.PG - 97-106

Authors: Hamner, S  Arumae, U  Li-Ying, Y  Sun, YF  Saarma, M  Lindholm, D 
Citation: Hamner S, etal., Mol Cell Neurosci 2001 Jan;17(1):97-106.
RGD ID: 625527
Pubmed: PMID:11161472   (View Abstract at PubMed)
DOI: DOI:10.1006/mcne.2000.0905   (Journal Full-text)

Neuronal cell death is in many cases regulated by competitive interactions between pro- and antiapoptotic proteins of the Bcl-2 family. In this study we have identified two splice variants of the rat proapoptotic molecule Bad, which differ in their carboxy-terminal regions. Both splice variants of Bad interacted with the antiapoptotic molecule Bcl-w as shown by yeast two-hybrid assay and by co-immunoprecipitation experiments from transfected cells. mRNA expression for the two variants of bad were detected in all neonatal and adult rat tissues tested. Overexpression of either of the two isoforms of Bad in nerve growth factor (NGF)-maintained sympathetic neurons by microinjection induced the cell death of these neurons, which was neutralized by co-expression of Bcl-w. Overexpression of Bcl-w in sympathetic neurons also counteracted death induced by NGF deprivation, which was not reduced by co-expression of either of the two Bad variants. The results suggest that Bcl-w, Bad-alpha, and Bad-beta may participate in the regulation of apoptosis in the sympathetic nervous system.AD - Department of Neuroscience and Neurobiology, UppsalaUniversity, Uppsala, 751 23, Sweden.FAU - Hamner, S

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPIBcl2l2 (Rattus norvegicus)625527 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Bad  (BCL2-associated agonist of cell death)


Additional Information