RGD Reference Report - Functional GABA(B) receptors expressed in cultured calvarial osteoblasts. - Rat Genome Database

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Functional GABA(B) receptors expressed in cultured calvarial osteoblasts.

Authors: Fujimori, S  Hinoi, E  Yoneda, Y 
Citation: Fujimori S, etal., Biochem Biophys Res Commun 2002 May 24;293(5):1445-52.
RGD ID: 625463
Pubmed: PMID:12054677   (View Abstract at PubMed)
DOI: DOI:10.1016/S0006-291X(02)00405-9   (Journal Full-text)

In immature and mature primary cultured rat calvarial osteoblasts, both mRNA and corresponding proteins were constitutively expressed for 2 splice variants of GABA(B) receptor (GABA(B)R) subunits but not for any known GABA(A) and GABA(C) receptor subunits. The agonist for GABA(B)R baclofen significantly inhibited cAMP formation induced by forskolin in a manner sensitive to the antagonist 2-hydroxysaclofen. Similar expression was seen with mRNA for GABA(B)R-1a and -1b splice variants in the murine calvarial osteoblast cell line MC3TC-E1 cells cultured for 7-21 days in vitro (DIV). In these MC3T3-E1 cells, baclofen not only inhibited the activity of alkaline phosphatase, but also exacerbated Ca2+ accumulation, throughout the culture period up to 28 DIV. These results suggest that GABA may play an unidentified role in mechanisms associated with cellular proliferation, differentiation, and/or development through functional GABA(B)R constitutively expressed in cultured osteoblasts.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
osteoblast differentiation  IMP 625463 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
G protein-coupled GABA receptor activity  IMP 625463 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gabbr1  (gamma-aminobutyric acid type B receptor subunit 1)


Additional Information