RGD Reference Report - Ca2+-dependent translocation of the calcyclin-binding protein in neurons and neuroblastoma NB-2a cells. - Rat Genome Database

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Ca2+-dependent translocation of the calcyclin-binding protein in neurons and neuroblastoma NB-2a cells.

Authors: Filipek, A  Jastrzebska, B  Nowotny, M  Kwiatkowska, K  Hetman, M  Surmacz, L  Wyroba, E  Kuznicki, J 
Citation: Filipek A, etal., J Biol Chem 2002 Jun 7;277(23):21103-9.
RGD ID: 625428
Pubmed: PMID:11927578   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M111010200   (Journal Full-text)

The calcyclin-binding protein (CacyBP) binds calcyclin (S100A6) at physiological levels of [Ca(2+)] and is highly expressed in brain neurons. Subcellular localization of CacyBP was examined in neurons and neuroblastoma NB-2a cells at different [Ca(2+)](i). Immunostaining indicates that CacyBP is present in the cytoplasm of unstimulated cultured neurons in which resting [Ca(2+)](i) is known to be approximately 50 nm. When [Ca(2+)](i) was increased to above 300 nm by KCl treatment, the immunostaining was mainly apparent as a ring around the nucleus. Such perinuclear localization of CacyBP was observed in untreated neuroblastoma NB-2a cells in which [Ca(2+)](i) is approximately 120 nm. An additional increase in [Ca(2+)](i) to above 300 nm by thapsigargin treatment did not change CacyBP localization. However, when [Ca(2+)](i) in NB-2a cells dropped to 70 nm, because of BAPTA/AM treatment, perinuclear localization was diminished. Ca(2+)-induced translocation of CacyBP was confirmed by immunogold electron microscopy and by fluorescence of NB-2a cells transfected with an EGFP-CacyBP vector. Recombinant CacyBP can be phosphorylated by protein kinase C in vitro. In untreated neuroblastoma NB-2a cells, CacyBP is phosphorylated on a serine residue(s), but exists in the dephosphorylated form in BAPTA/AM-treated cells. Thus, phosphorylation of CacyBP occurs in the same [Ca(2+)](i) range that leads to its perinuclear translocation.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to calcium ion  IEP 625428 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cacybp  (calcyclin binding protein)

Objects referenced in this article
Gene Cacybp calcyclin binding protein Mus musculus

Additional Information