RGD Reference Report - A novel S-adenosyl-L-methionine:arsenic(III) methyltransferase from rat liver cytosol. - Rat Genome Database

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A novel S-adenosyl-L-methionine:arsenic(III) methyltransferase from rat liver cytosol.

Authors: Lin, S  Shi, Q  Nix, FB  Styblo, M  Beck, MA  Herbin-Davis, KM  Hall, LL  Simeonsson, JB  Thomas, DJ 
Citation: Lin S, etal., J Biol Chem 2002 Mar 29;277(13):10795-803.
RGD ID: 625365
Pubmed: PMID:11790780   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M110246200   (Journal Full-text)

S-Adenosyl-l-methionine (AdoMet):arsenic(III) methyltransferase, purified from liver cytosol of adult male Fischer 344 rats, catalyzes transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. The kinetics of production of methylated arsenicals in reaction mixtures containing enzyme, AdoMet, dithiothreitol, glutathione (GSH), and arsenite are consistent with a scheme in which monomethylated arsenical produced from arsenite is the substrate for a second methylation reaction that yields dimethylated arsenical. The mRNA for this protein predicts a 369-amino acid residue protein (molecular mass 41056) that contains common methyltransferase sequence motifs. Its sequence is similar to Cyt19, a putative methyltransferase, expressed in human and mouse tissues. Reverse transcription-polymerase chain reaction detects S-adenosyl-l-methionine:arsenic(III) methyltransferase mRNA in rat tissues and in HepG2 cells, a human cell line that methylates arsenite and methylarsonous acid. S-Adenosyl-l-methionine:arsenic(III) methyltransferase mRNA is not detected in UROtsa cells, an immortalized human urothelial cell line that does not methylate arsenite. Because methylation of arsenic is a critical feature of its metabolism, characterization of this enzyme will improve our understanding of this metalloid's metabolism and its actions as a toxin and a carcinogen.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
As3mtRatarsonoacetate metabolic process involved_inIDA PMID:11790780UniProt 
As3mtRatresponse to arsenic-containing substance acts_upstream_of_or_withinIDA PMID:11790780MGI 
As3mtRattoxin metabolic process involved_inIDA PMID:11790780UniProt 

Cellular Component

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
As3mtRatcytosol located_inIDA PMID:11790780UniProt 

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
As3mtRatarsenite methyltransferase activity enablesIDA PMID:11790780MGI 
As3mtRatprotein methyltransferase activity  TAS  RGD 
As3mtRatS-adenosylmethionine-dependent methyltransferase activity  TAS  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
As3mtRathomocysteine metabolic pathway   TAS  RGD 
As3mtRatmethionine cycle/metabolic pathway   TAS  RGD 
Objects Annotated

Genes (Rattus norvegicus)
As3mt  (arsenite methyltransferase)

Objects referenced in this article
Gene AS3MT arsenite methyltransferase Homo sapiens

Additional Information