RGD Reference Report - Functional alterations of the Nppa promoter are linked to cardiac ventricular hypertrophy in WKY/WKHA rat crosses. - Rat Genome Database

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Functional alterations of the Nppa promoter are linked to cardiac ventricular hypertrophy in WKY/WKHA rat crosses.

Authors: Deschepper, CF  Masciotra, S  Zahabi, A  Boutin-Ganache, I  Picard, S  Reudelhuber, TL 
Citation: Deschepper CF, etal., Circ Res 2001 Feb 2;88(2):223-8.
RGD ID: 619659
Pubmed: PMID:11157676   (View Abstract at PubMed)

Cardiac left ventricular hypertrophy (LVH) is commonly associated with hypertension, but its variance is determined for more than 50% by blood pressure-independent genetic factors. Because it constitutes one of the most important risk factors for cardiovascular mortality, we have performed a genome-wide scan of the F2 progeny of crosses between inbred WKY and WKHA rats to detect quantitative trait loci (QTL) linked to cardiac mass. In addition to left ventricular mass (LVM), we also measured left ventricle (LV) concentration of atrial natriuretic factor (ANF), because we have previously established that there was a genetic link between these 2 traits in the same animal cross. We found 2 contiguous QTL on chromosome 5 that were linked to either LVM (logarithm of odds [LOD]=3.5) or log(n) (LV ANF) (LOD=12). The 1-LOD support intervals of both QTL shared a region overlapping the locus of natriuretic peptide precursor A (NPPA:) (ie, the ANF-coding gene). We found by sequencing 2 single nucleotide polymorphisms (SNPs) within the first 650 bp of the NPPA: minimal promoters of the genes from both strains. One of these SNPs increased the transcriptional activity of the NPPA: minimal promoter in transfected neonatal cardiomyocytes in keeping with the higher LV concentration of ANF observed in WKY versus WKHA rats. Taken together with the previous reports showing that ANF may protect cardiomyocytes against hypertrophy, our genetic data single out NPPA: as a strong candidate gene for the determination of LVM.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cm24RatLeft Ventricular Hypertrophy  IDA  RGD 
Lanf1RatLeft Ventricular Hypertrophy  IDA  RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cm24Ratheart left ventricle hypertrophy  QTM  RGD 
Lanf1Ratincreased circulating atrial natriuretic factor  QTM  RGD 
Objects Annotated

QTLs
Cm24  (Cardiac mass QTL 24)
Lanf1  (Left ventricular atrial natriuretic factor QTL 1)

Strains
WKHA/Cfd  (WKHA/Cfd)
WKY.WKHA-(D5Rat45-D5Rat245)/Cfd  (WKY.WKHA-(D5Rat45-D5Rat245)/Cfd)
WKY/Cfd  (WKY/Cfd)


Additional Information