RGD Reference Report - Rat endozepine-like peptide (ELP): cDNA cloning, genomic organization and tissue-specific expression. - Rat Genome Database

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Rat endozepine-like peptide (ELP): cDNA cloning, genomic organization and tissue-specific expression.

Authors: Pusch, W  Jahner, D  Spiess, AN  Ivell, R 
Citation: Pusch W, etal., Gene 1999 Jul 22;235(1-2):51-7.
RGD ID: 61789
Web Url: http://www.elsevier.com:80/cgi-bin/cas/tree/store/gene/cas_sub/browse/brow se.cgi?year=1999&volume=235&issue=1-2&aid=11936
Pubmed: PMID:10415332   (View Abstract at PubMed)

A cDNA encoding the rat homolog of the previously characterized murine endozepine-like peptide (ELP) was isolated by a PCR cloning strategy. Sequence comparison with the murine cDNA sequence revealed a conservation of the ELP primary structure between both rodent species with minor amino acid exchanges. We investigated the genomic organization of the rat ELP gene by genomic PCR. This indicated the presence of a single short intron of 451bp interrupting the 5' untranslated region. Tissue-dependent ELP expression was determined by Northern hybridization and semiquantitative RT-PCR. Northern hybridization showed an ELP specific transcript in both the male and the female gonad, but the level of ovarian ELP transcription was considerably lower than in the testis. RT-PCR analysis demonstrated a low and varying level of ELP background expression in all examined tissues. In contrast to the closely related ACBP gene, ELP shows a different genomic organization and a more regulated expression pattern, and may exert a specific function as a gonadal acyl-CoA pool former and transporter.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Dbil5Ratacyl-CoA metabolic process  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dbil5  (diazepam binding inhibitor-like 5)


Additional Information