RGD Reference Report - Involvement of G protein-coupled receptor kinase 5 in homologous desensitization of the thyrotropin receptor. - Rat Genome Database

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Involvement of G protein-coupled receptor kinase 5 in homologous desensitization of the thyrotropin receptor.

Authors: Nagayama, Y  Tanaka, K  Hara, T  Namba, H  Yamashita, S  Taniyama, K  Niwa, M 
Citation: Nagayama Y, etal., J Biol Chem 1996 Apr 26;271(17):10143-8.
RGD ID: 61618
Pubmed: PMID:8626574   (View Abstract at PubMed)

Homologous desensitization of G protein-coupled receptors involves agonist-dependent phosphorylation of receptors by G protein-coupled receptor kinases (GRKs). To identify GRK(s) that play a role in homologous desensitization of the thyrotropin (TSH) receptor, thyroid cDNA was amplified by polymerase chain reaction using degenerate oligonucleotide primers from highly conserved regions in GRK family. GRK5 is found in the predominant isoform expressed in the thyroid. Rat GRK5 cDNA was then isolated, which encodes a 590-amino acid protein with 95% homology to human and bovine homologs. Northern blot identified GRK5 mRNA of approximately 3, 8, and 10 kilobases with highest expression levels in lung > heart, kidney, colon > thyroid. In functional studies using a normal rat thyroid FRTL5 cells, overexpression of GRK5 by transfecting the plasmid capable of expressing the sense GRK5 RNA suppressed basal cAMP levels and augmented the extent of TSH receptor desensitization, whereas suppression of endogenous GRK5 expression by transfecting the antisense GRK5 construct increased basal cAMP levels and attenuated the extent of receptor desensitization. Although exogenously overexpressed GRK6 also enhanced TSH receptor desensitization, we conclude that GRK5, the predominant GRK isoform in the thyroid, appears to be mainly involved in homologous desensitization of the TSH receptor.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Grk5Ratdesensitization of G protein-coupled receptor signaling pathway  IMP  RGD 
Grk5Ratregulation of G protein-coupled receptor signaling pathway  IMP  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Grk5RatG protein-coupled receptor kinase activity  ICGO:0002029 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Grk5  (G protein-coupled receptor kinase 5)


Additional Information