RGD Reference Report - Rat sn-glycerol-3-phosphate acyltransferase: molecular cloning and characterization of the cDNA and expressed protein. - Rat Genome Database

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Rat sn-glycerol-3-phosphate acyltransferase: molecular cloning and characterization of the cDNA and expressed protein.

Authors: Ganesh Bhat, B  Wang, P  Kim, JH  Black, TM  Lewin, TM  Fiedorek, FT  Coleman, RA 
Citation: Ganesh Bhat B, etal., Biochim Biophys Acta 1999 Aug 18;1439(3):415-23.
RGD ID: 61613
Pubmed: PMID:10446428   (View Abstract at PubMed)

Rat mitochondrial glycerol-3-phosphate acyltransferase (GPAT) cDNA was cloned and characterized. We identified a cDNA containing an open reading frame of 828 amino acids that had an 89% homology with the coding region of the previously characterized mouse mitochondrial GPAT and a predicted amino acid sequence that was 96% identical. The rat 5' UTR was only 159 nucleotides, in contrast to the 926 nucleotide 5' UTR of the mouse cDNA and had an internal deletion of 167 nucleotides. GPAT was expressed in Sf21 insect cells, and specific inhibitors strongly suggest that, like the Escherichia coli GPAT, the recombinant mitochondrial GPAT and the mitochondrial GPAT isoform in rat liver contain critical serine, histidine, and arginine residues.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GpamRatglycerol-3-phosphate O-acyltransferase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gpam  (glycerol-3-phosphate acyltransferase, mitochondrial)


Additional Information