RGD Reference Report - Monoclonal antibodies to sucrase/isomaltase: probes for the study of postnatal development and biogenesis of the intestinal microvillus membrane. - Rat Genome Database

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Monoclonal antibodies to sucrase/isomaltase: probes for the study of postnatal development and biogenesis of the intestinal microvillus membrane.

Authors: Hauri, H P  Quaroni, A  Isselbacher, K J 
Citation: Hauri HP, etal., Proc Natl Acad Sci U S A. 1980 Nov;77(11):6629-33. doi: 10.1073/pnas.77.11.6629.
RGD ID: 597538471
Pubmed: PMID:6935673   (View Abstract at PubMed)
PMCID: PMC350340   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.77.11.6629   (Journal Full-text)

Two monoclonal antibodies, designated BB 3/34/12 and BB 5/8/40/90, have been produced to rat intestinal sucrase/isomaltase (SI) by the hybridoma technique using microvillus membranes as antigen. The BB 3/34/12 antibody was shown to be specific for the sucrase subunit. These antibodies provided new information regarding the biosynthesis and postnatal development of SI. In rat intestinal fetal transplants, SI was found exclusively in the form of an enzymatically active high molecular weight precursor, confirming our previous observations concerning the role of luminal proteases for the processing of SI in the microvillus membrane. The SI precursor, purified by affinity chromatography using the BB 3/34/12 antibody, had both sucrase and isomaltase activities, suggesting that a single precursor protein generates both sucrase and isomaltase subunits by proteolytic cleavage. The initial appearance of SI during normal postnatal development in the rat intestine was found to be confined to the cells present at the base of the villi. The same localization was observed after precocious induction of SI by cortisone acetate. In both cases, no immunofluorescence was observed in the crypts, suggesting that only the differentiated enterocyte is capable of synthesizing this enzyme. Even at the earliest times of appearance, newly synthesized SI was found almost completely split into its subunits, suggesting that the protease(s) responsible for the processing of the precursor in the microvillus membrane develop(s) in parallel with SI or earlier.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SiRatresponse to cortisone  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Si  (sucrase-isomaltase)


Additional Information