RGD Reference Report - The bile acid-sensitive ion channel (BASIC) mediates bile acid-dependent currents in bile duct epithelial cells.

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The bile acid-sensitive ion channel (BASIC) mediates bile acid-dependent currents in bile duct epithelial cells.
Authors:	Wiegreffe, Shari Löhrer, Daniel Wirtz, Monika Wiemuth, Dominik
Citation:	Wiegreffe S, etal., Pflugers Arch. 2021 Dec;473(12):1841-1850. doi: 10.1007/s00424-021-02622-2. Epub 2021 Sep 21.
RGD ID:	596936204
Pubmed:	PMID:34549327 (View Abstract at PubMed)
PMCID:	PMC8599227 (View Article at PubMed Central)
DOI:	DOI:10.1007/s00424-021-02622-2 (Journal Full-text)
The bile acid-sensitive ion channel (BASIC) is a member of the Deg/ENaC family of ion channels that is activated by bile acids. Despite the identification of cholangiocytes in the liver and unipolar brush cells in the cerebellum as sites of expression, the physiological function of BASIC in these cell types is not yet understood. Here we used a cholangiocyte cell line, normal rat cholangiocytes (NRCs), which expresses BASIC to study the role of the channel in epithelial transport using Ussing chamber experiments. Apical application of bile acids induced robust and transient increases in transepithelial currents that were carried by Na+ and partly blocked by the BASIC inhibitor diminazene. Genetic ablation of the BASIC gene in NRC using a CRISPR-cas9 approach resulted in a decrease of the bile acid-mediated response that matched the diminazene-sensitive current in NRC WT cells, suggesting that cholangiocytes respond to bile acids with a BASIC-mediated Na+ influx. Taken together, we have identified BASIC as a component of the cholangiocyte transport machinery, which might mediate a bile acid-dependent modification of the bile and thus control bile flux and composition.

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Biological Process

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Asic5	Rat	sodium ion import across plasma membrane	involved_in	IMP		PMID:34549327	UniProt

Cellular Component

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Asic5	Rat	apical plasma membrane	is_active_in	IDA		PMID:34549327	UniProt

Molecular Function

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Asic5	Rat	ligand-gated sodium channel activity	enables	IMP		PMID:34549327	UniProt

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Genes (Rattus norvegicus)

Asic5 (acid sensing ion channel subunit family member 5)

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The bile acid-sensitive ion channel (BASIC) mediates bile acid-dependent currents in bile duct epithelial cells.