RGD Reference Report - Cytostatic factor proteins are present in male meiotic cells and beta-nerve growth factor increases mos levels in rat late spermatocytes. - Rat Genome Database

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Cytostatic factor proteins are present in male meiotic cells and beta-nerve growth factor increases mos levels in rat late spermatocytes.

Authors: Perrard, Marie-Hélène  Chassaing, Emeric  Montillet, Guillaume  Sabido, Odile  Durand, Philippe 
Citation: Perrard MH, etal., PLoS One. 2009 Oct 5;4(10):e7237. doi: 10.1371/journal.pone.0007237.
RGD ID: 596933351
Pubmed: PMID:19802389   (View Abstract at PubMed)
PMCID: PMC2751818   (View Article at PubMed Central)
DOI: DOI:10.1371/journal.pone.0007237   (Journal Full-text)


BACKGROUND: In co-cultures of pachytene spermatocytes with Sertoli cells, beta-NGF regulates the second meiotic division by blocking secondary spermatocytes in metaphase (metaphase II), and thereby lowers round spermatid formation. In vertebrates, mature oocytes are arrested at metaphase II until fertilization, because of the presence of cytostatic factor (CSF) in their cytoplasm. By analogy, we hypothesized the presence of CSF in male germ cells.
METHODOLOGY/PRINCIPAL FINDINGS: We show here, that Mos, Emi2, cyclin E and Cdk2, the four proteins of CSF, and their respective mRNAs, are present in male rat meiotic cells; this was assessed by using Western blotting, immunocytochemistry and reverse transcriptase PCR. We measured the relative cellular levels of Mos, Emi2, Cyclin E and Cdk2 in the meiotic cells by flow cytometry and found that the four proteins increased throughout the first meiotic prophase, reaching their highest levels in middle to late pachytene spermatocytes, then decreased following the meiotic divisions. In co-cultures of pachytene spermatocytes with Sertoli cells, beta-NGF increased the number of metaphases II, while enhancing Mos and Emi2 levels in middle to late pachytene spermatocytes, pachytene spermatocytes in division and secondary spermatocytes.
CONCLUSION/SIGNIFICANCE: Our results suggest that CSF is not restricted to the oocyte. In addition, they reinforce the view that NGF, by enhancing Mos in late spermatocytes, is one of the intra-testicular factors which adjusts the number of round spermatids that can be supported by Sertoli cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Fbxo43Ratcellular response to nerve growth factor stimulus  IEP  RGD 
MosRatcellular response to nerve growth factor stimulus  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fbxo43  (F-box protein 43)
Mos  (MOS proto-oncogene, serine/threonine kinase)


Additional Information