RGD Reference Report - Adenoviral transfer of HSP-70 into pulmonary epithelium ameliorates experimental acute respiratory distress syndrome. - Rat Genome Database

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Adenoviral transfer of HSP-70 into pulmonary epithelium ameliorates experimental acute respiratory distress syndrome.

Authors: Weiss, YG  Maloyan, A  Tazelaar, J  Raj, N  Deutschman, CS 
Citation: Weiss YG, etal., J Clin Invest. 2002 Sep;110(6):801-6.
RGD ID: 5688755
Pubmed: PMID:12235111   (View Abstract at PubMed)
PMCID: PMC151132   (View Article at PubMed Central)
DOI: DOI:10.1172/JCI15888   (Journal Full-text)

The acute respiratory distress syndrome (ARDS) provokes three pathologic processes: unchecked inflammation, interstitial/alveolar protein accumulation, and destruction of pulmonary epithelial cells. The highly conserved heat shock protein HSP-70 can limit all three responses but is not appropriately expressed in the lungs after cecal ligation and double puncture (2CLP), a clinically relevant model of ARDS. We hypothesize that restoring expression of HSP-70 using adenovirus-mediated gene therapy will limit pulmonary pathology following 2CLP. We administered a vector containing the porcine HSP-70 cDNA driven by a CMV promoter (AdHSP) into the lungs of rats subjected to 2CLP or sham operation. Administration of AdHSP after either sham operation or 2CLP increased HSP-70 protein expression in lung tissue, as determined by immunohistochemistry and Western blot hybridization. Administration of AdHSP significantly attenuated interstitial and alveolar edema and protein exudation and dramatically decreased neutrophil accumulation, relative to a control adenovirus. CLP-associated mortality at 48 hours was reduced by half. Modulation of HSP-70 production reduces pathologic changes and may improve outcome in experimental ARDS.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
adult respiratory distress syndrome  ISOHspa4 (Rattus norvegicus)5688755; 5688755 RGD 
adult respiratory distress syndrome  IDA 5688755 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hspa4  (heat shock protein family A (Hsp70) member 4)

Genes (Mus musculus)
Hspa4  (heat shock protein 4)

Genes (Homo sapiens)
HSPA4  (heat shock protein family A (Hsp70) member 4)


Additional Information