RGD Reference Report - A proinflammatory chemokine, CCL3, sensitizes the heat- and capsaicin-gated ion channel TRPV1. - Rat Genome Database

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A proinflammatory chemokine, CCL3, sensitizes the heat- and capsaicin-gated ion channel TRPV1.

Authors: Zhang, N  Inan, S  Cowan, A  Sun, R  Wang, JM  Rogers, TJ  Caterina, M  Oppenheim, JJ 
Citation: Zhang N, etal., Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4536-41. Epub 2005 Mar 11.
RGD ID: 5688181
Pubmed: PMID:15764707   (View Abstract at PubMed)
PMCID: PMC555471   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.0406030102   (Journal Full-text)

Pain, a critical component of host defense, is one hallmark of the inflammatory response. We therefore hypothesized that pain might be exacerbated by proinflammatory chemokines. To test this hypothesis, CCR1 was cotransfected into human embryonic kidney (HEK)293 cells together with transient receptor potential vanilloid 1 (TRPV1), a cation channel required for certain types of thermal hyperalgesia. In these cells, capsaicin and anandamide induced Ca(2+) influx mediated by TRPV1. When CCR1:TRPV1/HEK293 cells were pretreated with CCL3, the sensitivity of TRPV1, as indicated by the Ca(2+) influx, was increased approximately 3-fold. RT-PCR analysis showed that a spectrum of chemokine and cytokine receptors is expressed in rat dorsal root ganglia (DRG). Immunohistochemical staining of DRG showed that CCR1 is coexpressed with TRPV1 in >85% of small-diameter neurons. CCR1 on DRG neurons was functional, as demonstrated by CCL3-induced Ca(2+) ion influx and PKC activation. Pretreatment with CCL3 enhanced the response of DRG neurons to capsaicin or anandamide. This sensitization was inhibited by pertussis toxin, U73122, or chelerythrine chloride, inhibitors of Gi-protein, phospholipase C, and protein kinase C, respectively. Intraplantar injection of mice with CCL3 decreased their hot-plate response latency. That a proinflammatory chemokine, by interacting with its receptor on small-diameter neurons, sensitizes TRPV1 reveals a previously undescribed mechanism of receptor cross-sensitization that may contribute to hyperalgesia during inflammation.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calcium ion transport involved_inIDA 5688181PMID:15764707UniProt 
cellular response to cytokine stimulus involved_inIDA 5688181PMID:15764707UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
external side of plasma membrane located_inIDA 5688181PMID:15764707UniProt 
neuronal cell body  IDA 5688181 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ligand-gated monoatomic ion channel activity enablesIDA 5688181PMID:15764707UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Ccr1  (C-C motif chemokine receptor 1)
Trpv1  (transient receptor potential cation channel, subfamily V, member 1)


Additional Information