RGD Reference Report - Developmental immunolocalization of heat shock protein 70 (HSP70) in epithelial cell of rat kidney. - Rat Genome Database

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Developmental immunolocalization of heat shock protein 70 (HSP70) in epithelial cell of rat kidney.

Authors: Kang, SS  Song, JH  Lee, MY  Kang, YH  Lim, SS  Ryu, SY  Jung, JY 
Citation: Kang SS, etal., Histol Histopathol. 2011 Nov;26(11):1363-73.
RGD ID: 5686902
Pubmed: PMID:21938673   (View Abstract at PubMed)

During renal development the cells in the medulla are exposed to elevated and variable interstitial osmolality. Heat shock protein 70 (HSP70) is a major molecular chaperone and plays an important role in the protection of cells in the renal medulla from high osmolality. The purpose of this study was to establish the time of immunolocalization and distribution of HSP70 in developing and adult rat kidney. In addition, changes in HSP70 immunolocalization following the infusion of furosemide were investigated. In adult animals, the HSP70 was expressed in the medullary thin ascending limb of Henle's loop (ATL) and inner medullary collecting duct (IMCD). In developing kidney, HSP70 immunoreactivity was first detected in the IMCD of the papillary tip on postnatal day 1. From four to 14 days of age, HSP70 was detected in the ATL after transformation from thick ascending limb, beginning at the papillary tip and ascending to the border between the outer and inner medulla. The immunolocalization of HSP70 in both the ATL and IMCD gradually increased during two weeks. The gradual increase in HSP70 was associated with an increase in its mRNA abundance. However, furosemide infusion resulted in significantly reduced HSP70 immunolocalization in the IMCD and ATL. These data demonstrated that the expression of HSP70 was closely correlated with changes in interstitial osmolality during the development of the kidney. We suggest that HSP70 protects ATL and IMCD cells in the inner medulla from the stress of high osmolality and may be involved in the transformation of the ATL of the long loop of Henle during renal development.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
kidney development  IEP 5686902 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hspa4  (heat shock protein family A (Hsp70) member 4)


Additional Information