RGD Reference Report - Differential proteomic characterization between normal peritoneal fluid and diabetic peritoneal dialysate. - Rat Genome Database

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Differential proteomic characterization between normal peritoneal fluid and diabetic peritoneal dialysate.

Authors: Wang, HY  Tian, YF  Chien, CC  Kan, WC  Liao, PC  Wu, HY  Su, SB  Lin, CY 
Citation: Wang HY, etal., Nephrol Dial Transplant. 2010 Jun;25(6):1955-63. Epub 2010 Jan 6.
RGD ID: 5509921
Pubmed: PMID:20054029   (View Abstract at PubMed)
DOI: DOI:10.1093/ndt/gfp696   (Journal Full-text)

BACKGROUND: Since the mechanism of comorbidity and mortality in peritoneal dialysis is unclear, a comparison of peritoneal dialysate and normal peritoneal fluid may provide clues to the biological and pathological processes involved in peritoneal damage. METHODS: Peritoneal dialysate and control samples were collected from five diabetes mellitus (DM) patients and two patients receiving laparoscopic cholecystectomy. Proteins were separated by two-dimensional gel electrophoresis (2D-GE). After image analysis, altered gel spots between these two sample groups were subjected to tryptic digestion and mass spectrometry analysis. The results were searched against the NCBI database. RESULTS: A total of 26 protein spots were considered altered in 2D-GE between the two sample groups. After western blotting confirmation, vitamin D-binding protein, haptoglobin and alpha-2-microglobulin were at higher levels in the DM samples, while complement C4-A and IGK@ protein were at lower levels compared to the control samples. CONCLUSION: The loss of vitamin D-binding protein, haptoglobin and alpha-2-microglobulin may be due to a change in the permeability of the peritoneal membrane to middle-sized proteins or leakage from peritoneal inflammation. Lower levels of complement C4-A in dialysate may shed light on the beginning of peritoneal membrane scleroses.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
diabetes mellitus  IEP 5509921protein:increased expression:ascitic fluidRGD 
diabetes mellitus  ISORDG:7369025509921; 5509921protein:increased expression:ascitic fluidRGD 

Objects Annotated

Genes (Rattus norvegicus)
Gc  (GC, vitamin D binding protein)

Genes (Mus musculus)
Gc  (vitamin D binding protein)

Genes (Homo sapiens)
GC  (GC vitamin D binding protein)


Additional Information