RGD Reference Report - Gel-based proteomics of liver cancer progression in rat. - Rat Genome Database

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Gel-based proteomics of liver cancer progression in rat.

Authors: Albrethsen, J  Miller, LM  Novikoff, PM  Angeletti, RH 
Citation: Albrethsen J, etal., Biochim Biophys Acta. 2011 Oct;1814(10):1367-76. Epub 2011 Jun 6.
RGD ID: 5509919
Pubmed: PMID:21683810   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbapap.2011.05.018   (Journal Full-text)

A significant challenge in proteomics biomarker research is to identify the changes that are of highest diagnostic interest, among the many unspecific aberrations associated with disease burden and inflammation. In the present study liver tissue specimens (n=18) from six experimental stages were collected from the resistant hepatocyte (RH) rat model of liver cancer and analyzed by 2D DIGE. The study included triplicates of regenerating liver, control "sham-operated" liver, three distinct premalignant stages and hepatomas. Out of 81 identified proteins two-thirds were differentially abundant in rat hepatomas compared to control rat liver and, secondly, the majority of proteins were also changed in precursor stages. This underscores the importance of adequate control samples in explorative cancer biomarker research. We confirm several proteomic changes previously identified in human hepatocellular carcinoma (HCC) and we identify novel candidate proteomic aberrations for further analysis in human HCC. In particular, increased levels of HSP70, HSP90, AKR1B1, AKR7A3, GCLM, ANXA5, VDBP, RGN and SULT1E1 were associated specifically with rat hepatomas, or with liver cancer progression in rat. In addition, we examine an integrated gel-based workflow for analysis of protein post-translational modifications (PTMs) and microtubule-association. We highlight differential PTM and localization of HSP60 as an interesting target for further analysis in liver cancer.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AKR1B1Humanhepatocellular carcinoma  ISORGD:2092protein:increased expression:liver (rat)RGD 
Akr1b1Rathepatocellular carcinoma  IEP protein:increased expression:liver (rat)RGD 
Akr1b1Mousehepatocellular carcinoma  ISORGD:2092protein:increased expression:liver (rat)RGD 
GCHumanhepatocellular carcinoma  ISORGD:2667protein:increased expression:liverRGD 
GcRathepatocellular carcinoma  IEP protein:increased expression:liverRGD 
GcMousehepatocellular carcinoma  ISORGD:2667protein:increased expression:liverRGD 
RGNHumanhepatocellular carcinoma  ISORGD:3560protein:decreased expression:liverRGD 
RgnRathepatocellular carcinoma  IEP protein:decreased expression:liverRGD 
RgnMousehepatocellular carcinoma  ISORGD:3560protein:decreased expression:liverRGD 

Objects Annotated

Genes (Rattus norvegicus)
Akr1b1  (aldo-keto reductase family 1 member B1)
Gc  (GC, vitamin D binding protein)
Rgn  (regucalcin)

Genes (Mus musculus)
Akr1b1  (aldo-keto reductase family 1 member B)
Gc  (vitamin D binding protein)
Rgn  (regucalcin)

Genes (Homo sapiens)
AKR1B1  (aldo-keto reductase family 1 member B)
GC  (GC vitamin D binding protein)
RGN  (regucalcin)


Additional Information